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验证数据展示

  • WB analysis using 20820-1-AP (same clone as 20820-1-PBS)

    WB analysis using 20820-1-AP (same clone as 20820-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 20820-1-AP (PTDSS1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20820-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 20820-1-AP (same clone as 20820-1-PBS)

    IF Staining of HeLa using 20820-1-AP (same clone as 20820-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using PTDSS1 antibody (20820-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 20820-1-PBS in a different storage buffer formulation.

  • IHC staining of human placenta using 20820-1-AP (same clone as 20820-1-PBS)

    IHC staining of human placenta using 20820-1-AP (same clone as 20820-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 20820-1-AP (PTDSS1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20820-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 20820-1-AP (same clone as 20820-1-PBS)

    IF Staining of HepG2 using 20820-1-AP (same clone as 20820-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using PTDSS1 antibody (20820-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 20820-1-PBS in a different storage buffer formulation.

PTDSS1 Polyclonal antibody, PBS Only

PTDSS1 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 20820-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

PSS1, EC:2.7.8.29, KIAA0024, Phosphatidylserine synthase 1, PSS-1

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

20820-1-PBS targets PTDSS1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag14170

Product name: Recombinant human PTDSS1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 394-473 aa of BC002376

Sequence: TTFLCLYGMIWYAEHYGHREKTYSECEDGTYSPEISWHHRKGTKGSEDSPPKHAGNNESHSSRRRNRHSKSKVTNGVGKK

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 phosphatidylserine synthase 1
别名 PSS1, EC:2.7.8.29, KIAA0024, Phosphatidylserine synthase 1, PSS-1
计算分子量 473 aa, 56 kDa
观测分子量38~40 kDa
GenBank蛋白编号BC002376
基因名称 PTDSS1
Gene ID (NCBI) 9791
RRIDAB_3669350
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP48651
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

PTDSS1 gene encodes the enzyme phosphatidylserine synthase 1 (PSS1), which catalyzes the production of phosphatidylserine (PS), a membrane phospholipid involved in different cellular processes such as development, cell signaling, apoptosis, and blood coagulation (PMID: 35224839). PTDSS1 is highly enriched in mitochondria-associated membranes (MAM) and is largely excluded from the bulk of the endoplasmic reticulum (ER). Low apparent molecular mass of 40 kDa compared with the size predicted was attributed to the exceptionally high content of hydrophobic amino acids in PSS1 (PMID: 10938271).

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