验证数据展示
经过测试的应用
| Positive WB detected in | Caco-2 cells, MCF-7 cells, MDA-MB-231 cells, mouse testis tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
33057-1-AP targets PTCD1 in WB, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human, mouse |
| 免疫原 |
CatNo: Ag37639 Product name: Recombinant human PTCD1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 201-595 aa of BC003688 Sequence: VWRLMLSLGLQPSRDSYNLLLVAARDCGLGDPQVASELLLKPREEATVLQPPVSRQRPRRTAQAKAGNLMSAMLHVEALERQLFLEPSQALGPPEPPEARVPGKAQPEVDTKAEPSHTAALTAVALKPPPVELEVNLLTPGAVPPTVVSFGTVTTPADRLALIGGLEGFLSKMAEHRQQPDIRTLTLLAEVVESGSPAESLLLALLDEHQVEADLTFFNTLVRKKSKLGDLEGAKALLPVLAKRGLVPNLQTFCNLAIGCHRPKDGLQLLTDMKKSQVTPNTHIYSALINAAIRKLNYTYLISILKDMKQNRVPVNEVVIRQLEFAAQYPPTFDRYQGKNTYLEKIDGFRAYYKQWLTVMPAEETPHPWQKFRTKPQGDQDTGKEADDGCALGGR 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | pentatricopeptide repeat domain 1 |
| 别名 | KIAA0632, Pentatricopeptide repeat-containing protein 1, mitochondrial |
| 计算分子量 | 79 kDa |
| 观测分子量 | 72 kDa |
| GenBank蛋白编号 | BC003688 |
| 基因名称 | PTCD1 |
| Gene ID (NCBI) | 26024 |
| RRID | AB_3742841 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity Purification |
| UNIPROT ID | O75127 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
PTCD1 protein (Pentatricopeptide Repeat Domain 1) is a nuclear gene-encoded protein located in the mitochondrial matrix. Its core function lies in regulating mitochondrial gene expression and the assembly of the respiratory chain. It specifically recognizes and binds to mitochondrial RNA (such as mRNA or pre-RNA) through its characteristic pentapeptide repeat sequence (PPR) motif, participating in RNA processing, stability maintenance, or translation processes, thereby being crucial for the assembly and function of mitochondrial oxidative phosphorylation complexes (especially complex I). Its deficiency is associated with mitochondrial dysfunction, neurodegenerative diseases, and cardiomyopathy and other pathological processes.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for PTCD1 antibody 33057-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
