验证数据展示
经过测试的应用
| Positive IHC detected in | human lung tissue, human heart tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
产品信息
20921-1-AP targets PTAFR in WB, IHC, ELISA applications and shows reactivity with human samples.
| 经测试应用 | IHC, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human |
| 文献引用反应性 | human, mouse |
| 免疫原 |
CatNo: Ag15128 Product name: Recombinant human PTAFR protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 245-342 aa of BC013816 Sequence: FVPHHVVQLPWTLAELGFQDSKFHQAINDAHQVTLCLLSTNCVLDPVIYCFLTKKFRKHLTEKFYSMRSSRKCSRATTDTVTEVVVPFNQIPGNSLKN 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | platelet-activating factor receptor |
| 别名 | PAF R, PAFR, PTAFR |
| 计算分子量 | 342 aa, 39 kDa |
| GenBank蛋白编号 | BC013816 |
| 基因名称 | PTAFR |
| Gene ID (NCBI) | 5724 |
| RRID | AB_2878766 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P25105 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| FC protocol for PTAFR antibody 20921-1-AP | Download protocol |
| IHC protocol for PTAFR antibody 20921-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Struct Mol Biol Structural basis for signal recognition and transduction by platelet-activating-factor receptor. | ||
Gut Pathog Virulence factors and mechanisms of paediatric pneumonia caused by Enterococcus faecalis |
