×
Use Able AI chat for product recommendations

验证数据展示

  • WB analysis using 14513-1-AP

    WB analysis using 14513-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14513-1-AP (PSPH antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IP experiment of HL-60 using 14513-1-AP

    IP experiment of HL-60 using 14513-1-AP

    IP result of anti-PSPH (IP:14513-1-AP, 4ug; Detection:14513-1-AP 1:300) with HL-60 cells lysate 3040ug.

  • IHC staining of human spleen using 14513-1-AP

    IHC staining of human spleen using 14513-1-AP

    Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 14513-1-AP (PSPH Antibody) at dilution of 1:100 (under 10x lens).

  • IHC staining of human spleen using 14513-1-AP

    IHC staining of human spleen using 14513-1-AP

    Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 14513-1-AP (PSPH Antibody) at dilution of 1:100 (under 40x lens).

  • WB analysis of U-87 MG using 14513-1-AP

    WB analysis of U-87 MG using 14513-1-AP

    WB result of PSPH antibody (14513-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PSPH transfected U-87 MG cells.

  • IF Staining of HepG2 using 14513-1-AP

    IF Staining of HepG2 using 14513-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using PSPH antibody (14513-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

  • FC experiment of HepG2 using 14513-1-AP

    FC experiment of HepG2 using 14513-1-AP

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug PSPH Polyclonal antibody (14513-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Intracellular Flow Cytometry Fixation & Permeabilization Buffer Kit (PF00019).

  • Featured Product
  • KD/KO Validated

PSPH Polyclonal antibody

PSPH Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA
Cat No. 14513-1-AP

产品说明书

引用文献(40)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, FC (Intra), IP, ELISA and More (1)

别名

EC:3.1.3.3, L-3-phosphoserine phosphatase, O-phosphoserine phosphohydrolase, PSP, PSPase

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive WB detected inA375 cells, rat liver tissue, U-87 MG cells, HL-60 cells, MCF-7 cells, SK-BR-3 cells
Positive IP detected inHL-60 cells
Positive IHC detected inhuman spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHepG2 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

14513-1-AP targets PSPH in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, FC (Intra), IP, ELISA Application Description
文献引用应用WB, IHC, IF, IP
经测试反应性 human, mouse, rat
文献引用反应性human, mouse
免疫原

CatNo: Ag5972

Product name: Recombinant human PSPH protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-225 aa of BC063614

Sequence: MVSHSELRKLFYSADAVCFDVDSTVIREEGIDELAKICGVEDAVSEMTRRAMGGAVPFKAALTERLALIQPSREQVQRLIAEQPPHLTPGIRELVSRLQERNVQVFLISGGFRSIVEHVASKLNIPATNVFANRLKFYFNGEYAGFDETQPTAESGGKGKVIKLLKEKFHFKKIIMIGDGATDMEACPPADAFIGFGGNVIRQQVKDNAKWYITDFVELLGELEE

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 phosphoserine phosphatase
别名 EC:3.1.3.3, L-3-phosphoserine phosphatase, O-phosphoserine phosphohydrolase, PSP, PSPase
计算分子量 25 kDa
观测分子量 25-28 kDa
GenBank蛋白编号BC063614
基因名称 PSPH
Gene ID (NCBI) 5723
RRIDAB_2171464
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP78330
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PSPH (phosphoserine phosphatase) is an enzyme, which is involved in the process of L-serine biosynthesis. PSPH mainly plays role in multiple aspects of cell behaviours such as proliferation and differentiation by producing precursors for the biosynthesis of diverse compounds including neurotransmitters, glycolipids and thymidine (PMID: 11237721, PMID: 19963421). Additionally, augmented PSPH level is correlated with the prognosis in multiple cancers including cutaneous squamous cell carcinoma (PMID: 21726982), breast cancer (PMID: 28931725), non-small cell lung cancer (PMID: 30662358), colorectal cancer (PMID: 24146633) and hepatocellular carcinoma (PMID: 25793315).

实验方案

Product Specific Protocols
FC protocol for PSPH antibody 14513-1-APDownload protocol
IF protocol for PSPH antibody 14513-1-APDownload protocol
IHC protocol for PSPH antibody 14513-1-APDownload protocol
IP protocol for PSPH antibody 14513-1-APDownload protocol
WB protocol for PSPH antibody 14513-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle

Science

mTORC1 induces purine synthesis through control of the mitochondrial tetrahydrofolate cycle.

Authors - Issam Ben-Sahra
View Article
humanWB,IHC

Mol Cancer

CircMYH9 drives colorectal cancer growth by regulating serine metabolism and redox homeostasis in a p53-dependent manner

Authors - Xin Liu
View Article
humanWB

Cell Metab

Epstein-Barr-Virus-Induced One-Carbon Metabolism Drives B Cell Transformation.

Authors - Liang Wei Wang
View Article
humanWB

Cell Metab

Serine metabolism antagonizes antiviral innate immunity by preventing ATP6V0d2-mediated YAP lysosomal degradation.

Authors - Long Shen
View Article
humanWB,IHC

Cell Res

cMyc-mediated activation of serine biosynthesis pathway is critical for cancer progression under nutrient deprivation conditions.

Authors - Linchong Sun
View Article
humanWB,IHC

Cancer Res

Targeting p300/CBP attenuates hepatocellular carcinoma progression through epigenetic regulation of metabolism.

Authors - Ling-Yan Cai
  • KD Validated
View Article
||
New chat

Able™

正在加载，请稍候...