验证数据展示
经过测试的应用
| Positive WB detected in | A431 cells, MCF-7 cells, MDA-MB-231 cells, SK-BR-3 cells |
| Positive IF/ICC detected in | A431 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
31201-1-AP targets PSMG4 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IF/ICC, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 |
CatNo: Ag35222 Product name: Recombinant human PSMG4 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-123 aa of NM_001128591.1 Sequence: MEGLVVAAGGDVSLHNFSARLWEQLVHFHVMRLTDSLFLWVGATPHLRNLAVAMCSRYDSIPVSTSLLGDTSDTTSTGLAQRLARKTNKQVFVSYNLQNTDSNFALLVENRIKEEMEAFPEKF 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | proteasome (prosome, macropain) assembly chaperone 4 |
| 别名 | bA506K6.2, C6orf86, hPAC4, PAC 4, PAC4 |
| 计算分子量 | 14 kDa |
| 观测分子量 | 13 kDa |
| GenBank蛋白编号 | NM_001128591.1 |
| 基因名称 | PSMG4 |
| Gene ID (NCBI) | 389362 |
| RRID | AB_3669898 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity Purification |
| UNIPROT ID | Q5JS54 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
PSMG4 (Proteasome assembly chaperone 4), also known as C6orf86. It is expected to be located in the nucleus and cytoplasm, which interacts with PSMG3 and asociates with alpha subunits of the 20S proteasome. The molecular weight of PSMG4 is 13 kDa.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for PSMG4 antibody 31201-1-AP | Download protocol |
| WB protocol for PSMG4 antibody 31201-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
