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验证数据展示

  • WB analysis using 15601-1-AP

    WB analysis using 15601-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15601-1-AP (PSMG3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis of HEK-293 using 15601-1-AP

    WB analysis of HEK-293 using 15601-1-AP

    WB result of PSMG3 antibody (15601-1-AP; 1:400; incubated at room temperature for 1.5 hours) with sh-Control and sh-PSMG3 transfected HEK-293 cells.

  • IHC staining of human stomach cancer using 15601-1-AP

    IHC staining of human stomach cancer using 15601-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 15601-1-AP (PSMG3 antibody) at dilution of 1:1600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  • KD/KO验证

PSMG3 Polyclonal antibody

PSMG3 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 15601-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, ELISA

别名

C7orf48, hPAC3, PAC 3, PAC3, PAC-3

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHEK-293 cells, Jurkat cells, K-562 cells, HeLa cells
Positive IHC detected inhuman stomach cancer tissue, human liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:800-1:3200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

15601-1-AP targets PSMG3 in WB, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag7984

Product name: Recombinant human PSMG3 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-122 aa of BC004308

Sequence: MEDTPLVISKQKTEVVCGVPTQVVCTAFSSHILVVVTQFGKMGTLVSLEPSSVASDVSKPVLTTKVLLGQDEPLIHVFAKNLVAFVSQEAGNRAVLLAVAVKDKSMEGLKALREVIRVCQVW

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 proteasome (prosome, macropain) assembly chaperone 3
别名 C7orf48, hPAC3, PAC 3, PAC3, PAC-3
计算分子量 13 kDa
观测分子量 13-18 kDa
GenBank蛋白编号BC004308
基因名称 PSMG3
Gene ID (NCBI) 84262
RRIDAB_2878156
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9BT73
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PSMG3, also named as C7orf48 and PAC3, is a chaperone protein which promotes assembly of the 20S proteasome. It may cooperate with PSMG1-PSMG2 heterodimers to orchestrate the correct assembly of proteasomes.

实验方案

Product Specific Protocols
IHC protocol for PSMG3 antibody 15601-1-APDownload protocol
WB protocol for PSMG3 antibody 15601-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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