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PSMG2 Polyclonal antibody

PSMG2 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 10959-1-AP

Print datasheet

Synonyms

CLAST3, HCCA3, HsT1707, MDS003, PAC 2, PAC2, PSMG2, TNFSF5IP1

验证数据展示

  • WB analysis using 10959-1-AP

    WB analysis using 10959-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 10959-1-AP (PSMG2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IHC staining of human prostate cancer using 10959-1-AP

    IHC staining of human prostate cancer using 10959-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer using 10959-1-AP (PSMG2 antibody) at dilution of 1:50 (under 10x lens).

经过测试的应用

Positive WB detected inHeLa cells, HepG2 cells, Jurkat cells
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

10959-1-AP targets PSMG2 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications IHC, WB, ELISA
Tested Reactivity human
Immunogen PSMG2 fusion protein Ag1400 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name proteasome (prosome, macropain) assembly chaperone 2
Synonyms CLAST3, HCCA3, HsT1707, MDS003, PAC 2, PAC2, PSMG2, TNFSF5IP1
Calculated Molecular Weight 29 kDa
Observed Molecular Weight 29 kDa
GenBank Accession NumberBC013356
Gene Symbol PSMG2
Gene ID (NCBI) 56984
RRIDAB_2171454
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ969U7
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Proteasome assembly chaperone 2 (PSMG2), also named as Hepatocellular carcinoma-susceptibility protein 3, Tumor necrosis factor superfamily member 5-induced protein 1. It has a function as a chaperone protein which promotes assembly of the 20S proteasome as part of a heterodimer with PSMG1. The PSMG1-PSMG2 heterodimer binds to the PSMA5 and PSMA7 proteasome subunits, promotes assembly of the proteasome alpha subunits into the heteroheptameric alpha ring and prevents alpha ring dimerization.It is widely expressed with highest levels in lung, brain and colon,moderately expressed in muscle, stomach, spleen and heart and weakly expressed in small intestine, pancreas and liver,highly expressed in hepatocellular carcinomas with low levels in surrounding liver tissue. Molecular mass of PSMG2 is 29kD.

实验方案

Product Specific Protocols
WB protocol for PSMG2 antibody 10959-1-APDownload protocol
IHC protocol for PSMG2 antibody 10959-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols