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PSMF1 Polyclonal antibody

PSMF1 Polyclonal Antibody for WB, ELISA
Cat No. 12941-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, ELISA

hPI31, PI31, PSMF1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存


经过测试的应用

Positive WB detected inHEK-293 cells, human kidney tissue, human heart tissue, Jurkat cells, Neuro-2a cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 2 publications below

产品信息

12941-1-AP targets PSMF1 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse, rat
文献引用反应性human
免疫原

CatNo: Ag4011

Product name: Recombinant human PSMF1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 122-271 aa of BC029836

Sequence: RTYKNSEELRSRIVSGIITPIHEQWEKANVSSPHREFPPATAREVDPLRIPPHHPHTSRQPPWCDPLGPFVVGGEDLDPFGPRRGGMIVDPLRSGFPRALIDPSSGLPNRLPPGAVPPGARFDPFGPIGTSPPGPNPDHLPPPGYDDMYL

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 proteasome (prosome, macropain) inhibitor subunit 1 (PI31)
别名 hPI31, PI31, PSMF1
计算分子量 30 kDa
观测分子量 30 kDa
GenBank蛋白编号BC029836
基因名称 PSMF1
Gene ID (NCBI) 9491
RRIDAB_2268855
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ92530
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PSMF1 is also named as PI31(proteasome (prosome, macropain) inhibitor subunit 1) and belongs to proteasome inhibitor PI31 family. PI31, which localizes at the nuclear envelope/endoplasmic reticulum membrane, acts as a selective modulator of the proteasome-mediated steps in MHC class I antigen processing(PMID:18495667). PI31 does not inhibit cell-surface transport of glycoproteins in general and it may serve to control immunoproteasome formation and may thereby maintain an intracellular balance between constitutive and immunoproteasomes(PMID:12374861).

实验方案

Product Specific Protocols
WB protocol for PSMF1 antibody 12941-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Mol Cell Biol

Interactome analysis reveals a novel role for RAD6 in the regulation of proteasome activity and localization in response to DNA damage.

Authors - Hongli An
humanWB

Mol Cell

Convergence of orphan quality control pathways at a ubiquitin chain-elongating ligase

Authors - Sara Carrillo Roas
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