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验证数据展示

  • WB analysis using 67338-1-Ig (same clone as 67338-1-PBS)

    WB analysis using 67338-1-Ig (same clone as 67338-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67338-1-Ig (PSMD9 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67338-1-PBS in a different storage buffer formulation.

  • Cytometric bead array sample test of MP51695-2

    Cytometric bead array sample test of MP51695-2

    Cytometric bead array sample test of MP51695-2, PSMD9 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 67338-2-PBS. Detection antibody: 67338-1-PBS.

  • WB analysis of A549 using 67338-1-Ig (same clone as 67338-1-PBS)

    WB analysis of A549 using 67338-1-Ig (same clone as 67338-1-PBS)

    WB result of PSMD9 antibody (67338-1-Ig; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PSMD9 transfected A549 cells. This data was developed using the same antibody clone with 67338-1-PBS in a different storage buffer formulation.

  • IF Staining of U2OS using 67338-1-Ig (same clone as 67338-1-PBS)

    IF Staining of U2OS using 67338-1-Ig (same clone as 67338-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using PSMD9 antibody (67338-1-Ig, Clone: 1H2G1 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 67338-1-PBS in a different storage buffer formulation.

  • FC experiment of HeLa using 67338-1-Ig (same clone as 67338-1-PBS)

    FC experiment of HeLa using 67338-1-Ig (same clone as 67338-1-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug PSMD9 Monoclonal antibody (67338-1-Ig, Clone:1H2G1) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.25 ug Mouse IgG1 isotype control Mouse McAb (66360-1-Ig, Clone: 1F8D3) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 67338-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP51695-2

    Cytometric bead array standard curve of MP51695-2

    Cytometric bead array standard curve of MP51695-2, PSMD9 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 67338-2-PBS. Detection antibody: 67338-1-PBS. Standard:Ag25654. Range: 0.781-100 ng/mL.

  • Featured Product
  • KD/KO Validated

PSMD9 Monoclonal antibody, PBS Only (Detector)

PSMD9 Monoclonal Antibody for WB, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA
Cat No. 67338-1-PBS

产品说明书

CloneNo. 1H2G1

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat

应用

WB, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

别名

1H2G1, 26S proteasome non-ATPase regulatory subunit 9, p27, Rpn4

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

67338-1-PBS targets PSMD9 as part of a matched antibody pair:

MP51695-2: 67338-2-PBS capture and 67338-1-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag25654

Product name: Recombinant human PSMD9 protein

Source: e coli.-derived, PET30a

Tag: 6*His

Domain: 1-102 aa of BC004213

Sequence: MSDEEARQSGGSSQAGVVTVSDVQELMRRKEEIEAQIKANYDVLESQKGIGMNEPLVDCEGYPRSDVDLYQVRTARHNIICLQNDHKAVMKQVEEALHQLHA

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 proteasome (prosome, macropain) 26S subunit, non-ATPase, 9
别名 1H2G1, 26S proteasome non-ATPase regulatory subunit 9, p27, Rpn4
计算分子量 27 kDa
观测分子量 25-30 kDa
GenBank蛋白编号BC004213
基因名称 PSMD9
Gene ID (NCBI) 5715
RRIDAB_2882596
偶联类型 Unconjugated
形式Liquid
纯化方式Protein G purification
UNIPROT IDO00233
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

PSMD9 is a ubiquitous protein of eukaryotic cells and is a chaperon of the 26S proteasome complex, which degrades ubiquitinated proteins in eukaryotic cells and contributes to the degradation of intracellular proteins into antigenic peptides for antigen presentation by MHC class I cells. The 26S mammalian base sub-complex involves three distinct modules which have ATPase subunits distinctly associated to three chaperones, one of which is PSMD9 regulating the modules assembly. The PSMD9 ubiquitous regulatory role within the proteasome implies its potential pleiotropic effects within different physio-pathological systems. PSMD9 is known to form a stable subcomplex with PSMC3 and PSMC6, two of the AAA-ATPases, assisting in the assembly of the 20S and 19S particles to form the holo complex.

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