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验证数据展示

  • WB analysis using 68188-1-Ig (same clone as 68188-1-PBS)

    WB analysis using 68188-1-Ig (same clone as 68188-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68188-1-Ig (PSMD7 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control. This data was developed using the same antibody clone with 68188-1-PBS in a different storage buffer formulation.

  • Cytometric bead array sample test of MP51841-1

    Cytometric bead array sample test of MP51841-1

    Cytometric bead array sample test of MP51841-1, PSMD7 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 68188-1-PBS. Detection antibody: 68188-2-PBS.

  • WB analysis of HeLa using 68188-1-Ig (same clone as 68188-1-PBS)

    WB analysis of HeLa using 68188-1-Ig (same clone as 68188-1-PBS)

    WB result of PSMD7 antibody (68188-1-Ig; 1:30000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PSMD7 transfected HeLa cells. This data was developed using the same antibody clone with 68188-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP51841-1

    Cytometric bead array standard curve of MP51841-1

    Cytometric bead array standard curve of MP51841-1, PSMD7 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 68188-1-PBS. Detection antibody: 68188-2-PBS. Standard:Ag9305. Range: 0.391-50 ng/mL.

  • Featured Product
  • KD/KO Validated

PSMD7 Monoclonal antibody, PBS Only (Capture)

PSMD7 Monoclonal Antibody for WB, Cytometric bead array, Indirect ELISA
Cat No. 68188-1-PBS

产品说明书

CloneNo. 3E10E7

宿主/亚型

Mouse / IgG1

种属反应性

human

应用

WB, Cytometric bead array, Indirect ELISA

别名

26S proteasome non-ATPase regulatory subunit 7, 3E10E7, MOV34, P40, Rpn8

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy PSMD7 Ready-To-Use IHC Kit. PSMD7 primary antibody included.

产品信息

68188-1-PBS targets PSMD7 as part of a matched antibody pair:

MP51841-1: 68188-1-PBS capture and 68188-2-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, Cytometric bead array, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag9305

Product name: Recombinant human PSMD7 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-319 aa of BC012606

Sequence: MPELAVQKVVVHPLVLLSVVDHFNRIGKVGNQKRVVGVLLGSWQKKVLDVSNSFAVPFDEDDKDDSVWFLDHDYLENMYGMFKKVNARERIVGWYHTGPKLHKNDIAINELMKRYCPNSVLVIIDVKPKDLGLPTEAYISVEEVHDDGTPTSKTFEHVTSEIGAEEAEEVGVEHLLRDIKDTTVGTLSQRITNQVHGLKGLNSKLLDIRSYLEKVATGKLPINHQIIYQLQDVFNLLPDVSLQEFVKAFYLKTNDQMVVVYLASLIRSVVALHNLINNKIANRDAEKKEGQEKEESKKDRKEDKEKDKDKEKSDVKKEE

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 proteasome (prosome, macropain) 26S subunit, non-ATPase, 7
别名 26S proteasome non-ATPase regulatory subunit 7, 3E10E7, MOV34, P40, Rpn8
计算分子量 324 aa, 37 kDa
观测分子量 37 kDa
GenBank蛋白编号BC012606
基因名称 PSMD7
Gene ID (NCBI) 5713
RRIDAB_2935278
偶联类型 Unconjugated
形式Liquid
纯化方式Protein G purification
UNIPROT IDP51665
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Proteasome 26S subunit, non-ATPase 7 (PSMD7), an ATP-independent component of the 19S regulatory subunit, is a member of the JAMM/MPN domain-associated metallopeptidase (JAMM) DUB family. PSMD7, as a core component of the 26S proteasome, is critical for the degradation of ubiquitinated proteins in the proteasome. PSMD7 arrests cell cycle in the G2/M phase during HIV infection. The molecular weight of PSMD7 is 37 kDa. (PMID: 34512150, 34234864)

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