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  • Featured Product
  • KD/KO Validated

PSMD7 Polyclonal antibody

PSMD7 Polyclonal Antibody for FC, IF/ICC, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

FC, IF/ICC, IHC, IP, WB, ELISA and More (1)

Conjugate

Unconjugated

Cat No : 16034-1-AP

Print datasheet

Synonyms

MOV34, Mov34 protein homolog, MOV34L, P40, Proteasome subunit p40, PSMD7, Rpn8, S12

验证数据展示

  • WB analysis using 16034-1-AP

    WB analysis using 16034-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16034-1-AP (PSMD7 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • WB analysis of HeLa using 16034-1-AP

    WB analysis of HeLa using 16034-1-AP

    WB result of PSMD7 antibody (16034-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PSMD7 transfected HeLa cells.

  • IP experiment of K-562 using 16034-1-AP

    IP experiment of K-562 using 16034-1-AP

    IP result of anti-PSMD7 (IP:16034-1-AP, 3ug; Detection:16034-1-AP 1:500) with K-562 cells lysate 2400ug.

  • FC experiment of HepG2 using 16034-1-AP

    FC experiment of HepG2 using 16034-1-AP

    1X10^6 HepG2 cells were stained with 0.2ug PSMD7 antibody (16034-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

  • IF Staining of HepG2 using 16034-1-AP

    IF Staining of HepG2 using 16034-1-AP

    Immunofluorescent analysis of HepG2 cells, using PSMD7 antibody 16034-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

  • IHC staining of human colon cancer using 16034-1-AP

    IHC staining of human colon cancer using 16034-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 16034-1-AP (PSMD7 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human colon cancer using 16034-1-AP

    IHC staining of human colon cancer using 16034-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 16034-1-AP (PSMD7 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of rat kidney using 16034-1-AP

    IHC staining of rat kidney using 16034-1-AP

    Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 16034-1-AP (PSMD7 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of rat kidney using 16034-1-AP

    IHC staining of rat kidney using 16034-1-AP

    Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 16034-1-AP (PSMD7 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse liver using 16034-1-AP

    IHC staining of mouse liver using 16034-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 16034-1-AP (PSMD7 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse liver using 16034-1-AP

    IHC staining of mouse liver using 16034-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 16034-1-AP (PSMD7 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inHeLa cells, K-562 cells, mouse liver tissue, rat liver tissue
Positive IP detected inK-562 cells
Positive IHC detected inhuman colon cancer tissue, human lung cancer tissue, mouse liver tissue, rat kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells
Positive FC detected inHepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)IF : 1:10-1:100
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

16034-1-AP targets PSMD7 in WB, IP, IF, FC, IHC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications FC, IF/ICC, IHC, IP, WB, ELISA
Cited ApplicationsWB, IF, CoIP
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen PSMD7 fusion protein Ag8905 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name proteasome (prosome, macropain) 26S subunit, non-ATPase, 7
Synonyms MOV34, Mov34 protein homolog, MOV34L, P40, Proteasome subunit p40, PSMD7, Rpn8, S12
Calculated Molecular Weight 324 aa, 37 kDa
Observed Molecular Weight 37 kDa
GenBank Accession NumberBC012606
Gene Symbol PSMD7
Gene ID (NCBI) 5713
RRIDAB_2170797
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP51665
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Proteasome 26S subunit, non-ATPase 7 (PSMD7), an ATP-independent component of the 19S regulatory subunit, is a member of the JAMM/MPN domain-associated metallopeptidase (JAMM) DUB family. PSMD7, as a core component of the 26S proteasome, is critical for the degradation of ubiquitinated proteins in the proteasome. PSMD7 arrests cell cycle in the G2/M phase during HIV infection. The molecular weight of PSMD7 is 37 kDa. (PMID: 34512150, 34234864)

实验方案

Product Specific Protocols
WB protocol for PSMD7 antibody 16034-1-APDownload protocol
IHC protocol for PSMD7 antibody 16034-1-APDownload protocol
IF protocol for PSMD7 antibody 16034-1-APDownload protocol
IP protocol for PSMD7 antibody 16034-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,CoIP

Int J Biol Sci

Deubiquitinase PSMD7 promotes the proliferation, invasion, and cisplatin resistance of gastric cancer cells by stabilizing RAD23B.

Authors - Jianjiang Wang
  • KD Validated
View Article
humanWB,IF,CoIP

Cell Signal

Deubiquitinating enzyme PSMD7 promotes bladder cancer development: Involvement of RAB1A stabilization

Authors - Jun Wang
View Article