验证数据展示
经过测试的应用
| Positive WB detected in | pig liver tissue, rabbit liver tissue, rat liver tissue, K-562 cells, HCT 116 cells |
| Positive IHC detected in | human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | A431 cells |
Planning an IHC experiment? We recommend our IHCeasy PSMD5 Ready-To-Use IHC Kit. PSMD5 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
67682-1-Ig targets PSMD5 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, pig, rat, rabbit, mouse samples.
| 经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
| 经测试反应性 | Human, pig, rat, rabbit, mouse |
| 免疫原 |
CatNo: Ag19298 Product name: Recombinant human PSMD5 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 154-504 aa of BC014478 Sequence: LTQAGLEALFESNLLDDLKSVMKTNDIVRYRVYELIIEISSVSPESLNYCTTSGLVTQLLRELTGEDVLVRATCIEMVTSLAYTHHGRQYLAQEGVIDQISNIIVGADSDPFSSFYLPGFVKFFGNLAVMDSPQQICERYPIFVEKVFEMIESQDPTMIGVAVDTVGILGSNVEGKQVLQKTGTRFERLLMRIGHQSKNAPVELKIRCLDAISSLLYLPPEQQTDDLLRMTESWFSSLSRDPLELFRGISSQPFPELHCAALKVFTAIANQPWAQKLMFNSPGFVEYVVDRSVEHDKASKDAKYELVKALANSKTIAEIFGNPNYLRLRTYLSEGPYYVKPVSTTAVEGAE 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG3 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | proteasome (prosome, macropain) 26S subunit, non-ATPase, 5 |
| 别名 | 26S protease subunit S5 basic, 26S proteasome subunit S5B, KIAA0072, PSMD5, S5B |
| 计算分子量 | 504 aa, 56 kDa |
| 观测分子量 | 50 kDa |
| GenBank蛋白编号 | BC014478 |
| 基因名称 | PSMD5 |
| Gene ID (NCBI) | 5711 |
| RRID | AB_2882875 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | Q16401 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for PSMD5 antibody 67682-1-Ig | Download protocol |
| IHC protocol for PSMD5 antibody 67682-1-Ig | Download protocol |
| WB protocol for PSMD5 antibody 67682-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
