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验证数据展示

  • WB analysis using RMX00058

    WB analysis using RMX00058

    Various lysates were subjected to SDS PAGE followed by western blot with RMX00058 (PSMD4 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse testis using RMX00058

    IHC staining of mouse testis using RMX00058

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using RMX00058 (PSMD4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PSMD4 antibody included
  • IF Staining of HepG2 using RMX00058

    IF Staining of HepG2 using RMX00058

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using PSMD4 antibody (RMX00058) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

Multi-rAb® PSMD4 Multi-Recombinant antibody

PSMD4 Multi-Recombinant Antibody for WB, IHC, IF/ICC, ELISA
Cat No. RMX00058

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, ELISA

别名

26S proteasome non-ATPase regulatory subunit 4, AF, ASF, MCB1, Multiubiquitin chain-binding protein

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inRaji cells, K-562 cells, RAW 264.7 cells, rat spleen tissue
Positive IHC detected inmouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy PSMD4 Ready-To-Use IHC Kit. PSMD4 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

RMX00058 targets PSMD4 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

Recombinant protein

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Multi-Recombinant
产品类型 Antibody
全称 proteasome (prosome, macropain) 26S subunit, non-ATPase, 4
别名 26S proteasome non-ATPase regulatory subunit 4, AF, ASF, MCB1, Multiubiquitin chain-binding protein
计算分子量 41 kDa
观测分子量 50 kDa
GenBank蛋白编号BC002365
基因名称 PSMD4
Gene ID (NCBI) 5710
RRIDAB_3750109
偶联类型 Unconjugated
形式Liquid
纯化方式N/A
UNIPROT IDP55036
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PSMD4, also named as MCB1, AF or ASF, belongs to the proteasome subunit S5A family. PSMD4 is a 50-kD subunit of the regulatory complex of the 26S proteasome. It binds and presumably selects ubiquitin-conjugates for destruction. PSMD4 displays selectivity for longer polyubiquitin chains. It modulates intestinal fluid secretion. PSMD4 is exposed on the sperm acrosomal surface, a notion that was further supported by the binding of non-cell permeant, biotinylated proteasomal inhibitor ZL3VS to the sperm acrosome. It is involved in the sperm-ZP penetration during fertilization. (PMID: 20526895)

实验方案

Product Specific Protocols
IF protocol for PSMD4 antibody RMX00058Download protocol
IHC protocol for PSMD4 antibody RMX00058Download protocol
WB protocol for PSMD4 antibody RMX00058Download protocol
Standard Protocols
Click here to view our Standard Protocols
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