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验证数据展示

  • WB analysis using 86558-1-RR (same clone as 86558-1-PBS)

    WB analysis using 86558-1-RR (same clone as 86558-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 86558-1-RR (PSMD4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86558-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse testis using 86558-1-RR (same clone as 86558-1-PBS)

    IHC staining of mouse testis using 86558-1-RR (same clone as 86558-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 86558-1-RR (PSMD4 antibody) at dilution of 1:600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86558-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PSMD4 antibody included
  • IF Staining of HeLa using 86558-1-RR (same clone as 86558-1-PBS)

    IF Staining of HeLa using 86558-1-RR (same clone as 86558-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PSMD4 antibody (86558-1-RR, Clone: 251460G8 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 86558-1-PBS in a different storage buffer formulation.

PSMD4 Recombinant monoclonal antibody, PBS Only

PSMD4 Uni-rAb® Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 86558-1-PBS

产品说明书

CloneNo. 251460G8

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

26S proteasome non-ATPase regulatory subunit 4, AF, ASF, MCB1, Multiubiquitin chain-binding protein

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy PSMD4 Ready-To-Use IHC Kit. PSMD4 primary antibody included.

产品信息

86558-1-PBS targets PSMD4 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag6691

Product name: Recombinant human PSMD4 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-377 aa of BC002365

Sequence: MVLESTMVCVDNSEYMRNGDFLPTRLQAQQDAVNIVCHSKTRSNPENNVGLITLANDCEVLTTLTPDTGRILSKLHTVQPKGKITFCTGIRVAHLALKHRQGKNHKMRIIAFVGSPVEDNEKDLVKLAKRLKKEKVNVDIINFGEEEVNTEKLTAFVNTLNGKDGTGSHLVTVPPGPSLADALISSPILAGEGGAMLGLGASDFEFGVDPSADPELALALRVSMEEQRQRQEEEARRAAAASAAEAGIATTGTEDSDDALLKMTISQQEFGRTGLPDLSSMTEEEQIAYAMQMSLQGAEFGQAESADIDASSAMDTSEPAKEEDDYDVMQDPEFLQSVLENLPGVDPNNEAIRNAMGSLASQATKDGKKDKKEEDKK

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 proteasome (prosome, macropain) 26S subunit, non-ATPase, 4
别名 26S proteasome non-ATPase regulatory subunit 4, AF, ASF, MCB1, Multiubiquitin chain-binding protein
计算分子量 41 kDa
观测分子量 50 kDa
GenBank蛋白编号BC002365
基因名称 PSMD4
Gene ID (NCBI) 5710
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP55036
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

PSMD4, also named as MCB1, AF or ASF, belongs to the proteasome subunit S5A family. PSMD4 is a 50-kD subunit of the regulatory complex of the 26S proteasome. It binds and presumably selects ubiquitin-conjugates for destruction. PSMD4 displays selectivity for longer polyubiquitin chains. It modulates intestinal fluid secretion. PSMD4 is exposed on the sperm acrosomal surface, a notion that was further supported by the binding of non-cell-permeant, biotinylated proteasomal inhibitor ZL3VS to the sperm acrosome. It is involved in the sperm-ZP penetration during fertilization. (PMID: 20526895)

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