验证数据展示
经过测试的应用
| Positive WB detected in | A549 cells, human brain tissue, human kidney tissue, mouse brain tissue, HEK-293 cells, mouse kidney tissue, rat kidney tissue |
| Positive IP detected in | mouse kidney tissue |
| Positive IHC detected in | human colon cancer tissue, human breast cancer tissue, human small intestine tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 4 publications below |
| IF | See 1 publications below |
产品信息
12054-1-AP targets PSMD3 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IP, ELISA Application Description |
| 文献引用应用 | WB, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human |
| 免疫原 |
CatNo: Ag2684 Product name: Recombinant human PSMD3 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 235-534 aa of BC012302 Sequence: LRTATLRHDADGQATLLNLLLRNYLHYSLYDQAEKLVSKSVFPEQANNNEWARYLYYTGRIKAIQLEYSVARRTMTNALRKAPQHTAVGFKQTVHKLLIVVELLLGEIPDRLQFRQPSLKRSLMPYFLLTQAVRTGNLAKFNQVLDQFGEKFQADGTYTLIIRLRHNVIKTGVRMISLSYSRISLADIAQKLQLDSPEDAEFIVAKAIRDGVIEASINHEKGYVQSKEMIDIYSTREPQLAFHQRISFCLDIHNMSVKAMRFPPKSYNKDLESAEERREREQQDLEFAKEMAEDDDDSFP 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | proteasome (prosome, macropain) 26S subunit, non-ATPase, 3 |
| 别名 | 26S proteasome non-ATPase regulatory subunit 3, P58, RPN3, S3, TSTA2 |
| 计算分子量 | 534 aa, 61 kDa |
| 观测分子量 | 61 kDa |
| GenBank蛋白编号 | BC012302 |
| 基因名称 | PSMD3 |
| Gene ID (NCBI) | 5709 |
| RRID | AB_2170484 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | O43242 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
PSMD3, encoding proteasome (prosome, macropain) 26S subunit, non-ATPase 3, a member of the 26S proteasome family. PSMD3 is expressed in most of the tissues according to the RNA-Seq database. It has been reported that PSMD3 genotypes are associated with white blood cell (WBC) and neutrophil counts and have interactions with dietary fatty acids and carbohydrates on glucose-related traits. PSMD3 is located at ch17q21 and encodes a protein with a molecular weight of 61 kDa (PMID: 31013812).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for PSMD3 antibody 12054-1-AP | Download protocol |
| IHC protocol for PSMD3 antibody 12054-1-AP | Download protocol |
| IP protocol for PSMD3 antibody 12054-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Protein Cell DSSylation, a novel protein modification targets proteins induced by oxidative stress, and facilitates their degradation in cells. | ||
Cell Death Dis ALG-2 couples T cell activation and apoptosis by regulating proteasome activity and influencing MCL1 stability. | ||
Cell Mol Life Sci The contribution of proteasomal impairment to autophagy activation by C9orf72 poly-GA aggregates |
