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PSMD13 Monoclonal antibody

PSMD13 Monoclonal Antibody for IF/ICC, IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat, rabbit, pig, chicken

Applications

IF/ICC, IHC, WB, ELISA

Conjugate

Unconjugated

CloneNo.

2G10G6

Cat No : 67807-1-Ig

Print datasheet

Synonyms

HSPC027, p40.5, PSMD13, Rpn9, S11

验证数据展示

  • WB analysis using 67807-1-Ig

    WB analysis using 67807-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67807-1-Ig (PSMD13 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis using 67807-1-Ig

    WB analysis using 67807-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67807-1-Ig (PSMD13 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • IHC staining of human pancreas cancer using 67807-1-Ig

    IHC staining of human pancreas cancer using 67807-1-Ig

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 67807-1-Ig (PSMD13 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PSMD13 antibody included
  • IHC staining of human pancreas cancer using 67807-1-Ig

    IHC staining of human pancreas cancer using 67807-1-Ig

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 67807-1-Ig (PSMD13 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PSMD13 antibody included
  • IHC staining of human lung cancer using 67807-1-Ig

    IHC staining of human lung cancer using 67807-1-Ig

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67807-1-Ig (PSMD13 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PSMD13 antibody included
  • IHC staining of human lung cancer using 67807-1-Ig

    IHC staining of human lung cancer using 67807-1-Ig

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67807-1-Ig (PSMD13 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PSMD13 antibody included
  • IF Staining of HepG2 using 67807-1-Ig

    IF Staining of HepG2 using 67807-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using PSMD13 antibody (67807-1-Ig, Clone: 2G10G6 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

经过测试的应用

Positive WB detected inHCT 116 cells, SMMC-7721 cells, HepG2 cells, HuH-7 cells, mouse liver tissue, NIH/3T3 cells, HSC-T6 cells, K-562 cells, Jurkat cells, HEK-293 cells, HeLa cells, L02 cells, A549 cells, Calu-3 cells, rat liver tissue, rabbit liver tissue, pig liver, chicken liver
Positive IHC detected inhuman pancreas cancer tissue, human lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy PSMD13 Ready-To-Use IHC Kit. PSMD13 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:2000-1:8000
Immunofluorescence (IF)IF : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67807-1-Ig targets PSMD13 in WB, IF, IHC, ELISA applications and shows reactivity with Human, mouse, rat, rabbit, pig, chicken samples.

Tested Applications IF/ICC, IHC, WB, ELISA
Tested Reactivity Human, mouse, rat, rabbit, pig, chicken
Immunogen PSMD13 fusion protein Ag7857 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name proteasome (prosome, macropain) 26S subunit, non-ATPase, 13
Synonyms HSPC027, p40.5, PSMD13, Rpn9, S11
Calculated Molecular Weight 43 kDa
Observed Molecular Weight43 kDa
GenBank Accession NumberBC001100
Gene Symbol PSMD13
Gene ID (NCBI) 5719
RRIDAB_2918570
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ9UNM6
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for PSMD13 antibody 67807-1-IgDownload protocol
IHC protocol for PSMD13 antibody 67807-1-IgDownload protocol
IF protocol for PSMD13 antibody 67807-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols