验证数据展示
经过测试的应用
Positive WB detected in | HEK-293 cells, K-562 cells, Jurkat cells |
Positive IP detected in | Jurkat cells |
Positive IHC detected in | mouse testis tissue, rat testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | HepG2 cells |
推荐稀释比
应用 | 推荐稀释比 |
---|---|
Western Blot (WB) | WB : 1:500-1:2000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:400-1:1600 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
11339-1-AP targets PSMC3IP in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
经测试应用 | WB, IHC, IF/ICC, IP, ELISA Application Description |
文献引用应用 | WB, IF |
经测试反应性 | human, mouse, rat |
文献引用反应性 | human, mouse |
免疫原 | PSMC3IP fusion protein Ag1883 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | PSMC3 interacting protein |
别名 | PSMC3-interacting protein, PSMC3 interacting protein, Proteasome 26S ATPase subunit 3-interacting protein, HOP2, Homologous-pairing protein 2 homolog |
计算分子量 | 25 kDa |
观测分子量 | 24-29 kDa |
GenBank蛋白编号 | BC008792 |
基因名称 | PSMC3IP |
Gene ID (NCBI) | 29893 |
RRID | AB_2172642 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Antigen affinity purification |
UNIPROT ID | Q9P2W1 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
PSMC3IP, also called HOP2, encodes a protein that functions in meiotic recombination. It is a subunit of the PSMC3IP/MND1 complex, which interacts with PSMC3/TBP1 to stimulate DMC1- and RAD51-mediated strand exchange during meiosis. The protein encoded by this gene can also co-activate ligand-driven transcription mediated by estrogen, androgen, glucocorticoid, progesterone, and thyroid nuclear receptors. Mutations in this gene cause XX female gonadal dysgenesis.
实验方案
Product Specific Protocols | |
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WB protocol for PSMC3IP antibody 11339-1-AP | Download protocol |
IHC protocol for PSMC3IP antibody 11339-1-AP | Download protocol |
IF protocol for PSMC3IP antibody 11339-1-AP | Download protocol |
IP protocol for PSMC3IP antibody 11339-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Cell Interchromosomal Homology Searches Drive Directional ALT Telomere Movement and Synapsis.
| ||
Nucleic Acids Res RNase H1 facilitates recombinase recruitment by degrading DNA-RNA hybrids during meiosis | ||
Oncotarget A study of meiomitosis and novel pathways of genomic instability in cutaneous T-cell lymphomas (CTCL). |