• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

PSMC3 Polyclonal antibody

PSMC3 Polyclonal Antibody for IF/ICC, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

IF/ICC, IHC, IP, WB,ELISA

Conjugate

Unconjugated

Cat No : 24142-1-AP

Print datasheet

Synonyms

Proteasome subunit P50, PSMC3, Tat binding protein 1, TBP 1, TBP1

验证数据展示

  • WB analysis using 24142-1-AP

    WB analysis using 24142-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 24142-1-AP (PSMC3 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • IP experiment of HeLa using 24142-1-AP

    IP experiment of HeLa using 24142-1-AP

    IP result of anti-PSMC3 (IP:24142-1-AP, 4ug; Detection:24142-1-AP 1:800) with HeLa cells lysate 3200ug.

  • IHC staining of human ovary tumor using 24142-1-AP

    IHC staining of human ovary tumor using 24142-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 24142-1-AP (PSMC3 Antibody) at dilution of 1:50 (under 10x lens).

    Ready-to-use IHC kits available with PSMC3 antibody included
  • IHC staining of human ovary tumor using 24142-1-AP

    IHC staining of human ovary tumor using 24142-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 24142-1-AP (PSMC3 Antibody) at dilution of 1:50 (under 40x lens).

    Ready-to-use IHC kits available with PSMC3 antibody included
  • IF Staining of HEK-293 using 24142-1-AP

    IF Staining of HEK-293 using 24142-1-AP

    Immunofluorescent analysis of HEK-293 cells using 24142-1-AP (PSMC3 antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

经过测试的应用

Positive WB detected inHEK-293 cells, A431 cells, HeLa cells, HepG2 cells, Jurkat cells, MCF-7 cells, T-47D cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman ovary tumor tissue, human tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHEK-293 cells, HepG2 cells
Planning an IHC experiment? We recommend our IHCeasy PSMC3 Ready-To-Use IHC Kit. PSMC3 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 2 publications below

产品信息

The immunogen of 24142-1-AP is PSMC3 Fusion Protein expressed in E. coli.

Tested Applications IF/ICC, IHC, IP, WB,ELISA
Cited ApplicationsWB
Tested Reactivity human
Cited Reactivityhuman, mouse
Immunogen PSMC3 fusion protein Ag21408 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name proteasome (prosome, macropain) 26S subunit, ATPase, 3
Synonyms Proteasome subunit P50, PSMC3, Tat binding protein 1, TBP 1, TBP1
Calculated Molecular Weight 49 kDa
Observed Molecular Weight 49 kDa
GenBank Accession NumberBC008713
Gene Symbol PSMC3
Gene ID (NCBI) 5702
RRIDAB_2879440
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen Affinity purified
UNIPROT IDP17980
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PSMC3(26S protease regulatory subunit 6A) is also named as TBP1, Proteasome 26S subunit ATPase 3 and belongs to the AAA ATPase family. The 26S protease is involved in the ATP-dependent degradation of ubiquitinated proteins. It is a multifunctional protein, component of the regulatory subunit of the proteasome, involved in different cellular processes.

实验方案

Product Specific Protocols
WB protocol for PSMC3 antibody 24142-1-APDownload protocol
IHC protocol for PSMC3 antibody 24142-1-APDownload protocol
IF protocol for PSMC3 antibody 24142-1-APDownload protocol
IP protocol for PSMC3 antibody 24142-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

ChemMedChem

Pyrazolones Activate the Proteasome by Gating Mechanisms and Protect Neuronal Cells from β-Amyloid Toxicity.

Authors - Anna Maria Santoro
View Article
human,mouseWB

Elife

A cleaved METTL3 potentiates the METTL3-WTAP interaction and breast cancer progression

Authors - Chaojun Yan
View Article