验证数据展示
经过测试的应用
| Positive WB detected in | HL-60 cells, Ramos cells, Daudi cells, Raji cells, mouse spleen tissue, rat spleen tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
87013-1-RR targets PSMB9 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human, mouse, rat |
| 免疫原 |
CatNo: Ag6048 Product name: Recombinant human PSMB9 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-219 aa of BC065513 Sequence: MLRAGAPTGDLPRAGEVHTGTTIMAVEFDGGVVMGSDSRVSAGEAVVNRVFDKLSPLHERIYCALSGSAADAQAVADMAAYQLELHGIELEEPPLVLAAANVVRNISYKYREDLSAHLMVAGWDQREGGQVYGTLGGMLTRQPFAIGGSGSTFIYGYVDAAYKPGMSPEECRRFTTDAIALAMSRDGSSGGVIYLVTITAAGVDHRVILGNELPKFYDE 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | proteasome (prosome, macropain) subunit, beta type, 9 (large multifunctional peptidase 2) |
| 别名 | LMP2, EC:3.4.25.1, Proteasome subunit beta type-9, Proteasome subunit beta-1i, PSMB6i |
| 计算分子量 | 23 kDa |
| 观测分子量 | 20-23 kDa |
| GenBank蛋白编号 | BC065513 |
| 基因名称 | PSMB9 |
| Gene ID (NCBI) | 5698 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P28065 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
PSMB9, also named as LMP2, PSMB6i and RING12, belongs to the peptidase T1B family. The proteasome is an essential component of the cellular protein degradation machinery . It can be isolated as a 20 S particle containing more than 15 different subunits ranging in molecular masses from 20-35 kDa. The proteasome can also be isolated as part of a higher molecular mass particle of 26 S which has been shown to rapidly degrade both ubiquitinated and nonubiquitinated proteins in a ATP-dependent fashion. Replacement of PSMB6 by PSMB9 increases the capacity of the immunoproteasome to cleave model peptides after hydrophobic and basic residues. High expression of PSMB9 associated with the poor prognosis of patients with NPC. (PMID: 1429565)
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for PSMB9 antibody 87013-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
