验证数据展示
经过测试的应用
| Positive WB detected in | Raji cells, rat spleen tissue, Ramos cells, Daudi cells, HL-60 cells, THP-1 cells |
| Positive IHC detected in | human colon cancer tissue, human spleen tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Planning an IHC experiment? We recommend our IHCeasy PSMB9 Ready-To-Use IHC Kit. PSMB9 primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:300-1:1200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
67748-1-Ig targets PSMB9 in WB, IHC, ELISA applications and shows reactivity with Human, rat samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 经测试反应性 | Human, rat |
| 免疫原 |
CatNo: Ag6802 Product name: Recombinant human PSMB9 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-219 aa of BC065513 Sequence: MLRAGAPTGDLPRAGEVHTGTTIMAVEFDGGVVMGSDSRVSAGEAVVNRVFDKLSPLHERIYCALSGSAADAQAVADMAAYQLELHGIELEEPPLVLAAANVVRNISYKYREDLSAHLMVAGWDQREGGQVYGTLGGMLTRQPFAIGGSGSTFIYGYVDAAYKPGMSPEECRRFTTDAIALAMSRDGSSGGVIYLVTITAAGVDHRVILGNELPKFYDE 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG2a |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | proteasome (prosome, macropain) subunit, beta type, 9 (large multifunctional peptidase 2) |
| 别名 | beta1i, LMP2, Low molecular mass protein 2, Macropain chain 7, Proteasome chain 7, Proteasome subunit beta 1i, Proteasome subunit beta type 9, PSMB6i, PSMB9, RING12 |
| 计算分子量 | 23 kDa |
| 观测分子量 | 20-23 kDa |
| GenBank蛋白编号 | BC065513 |
| 基因名称 | PSMB9 |
| Gene ID (NCBI) | 5698 |
| RRID | AB_2918517 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P28065 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for PSMB9 antibody 67748-1-Ig | Download protocol |
| WB protocol for PSMB9 antibody 67748-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
