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验证数据展示

  • WB analysis using 86241-1-RR

    WB analysis using 86241-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 86241-1-RR (PSMB8 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 86241-1-RR

    IF Staining of HeLa using 86241-1-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PSMB8 antibody (86241-1-RR, Clone: 250865C7 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Affinity and Kinetic Characterization of 86241-1-RR

    Affinity and Kinetic Characterization of 86241-1-RR

    Biolayer interferometry (BLl) kinetic assays of 86241-1-RR against Human PSMB8 were performed. The affinity constant is below 1 pM.

PSMB8 Recombinant monoclonal antibody

PSMB8 Uni-rAb® Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 86241-1-RR

产品说明书

CloneNo. 250865C7

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, ELISA

别名

EC:3.4.25.1, LMP7, Proteasome subunit beta type 8, Proteasome subunit beta type-8, Proteasome subunit beta-5i

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inJurkat cells, HuT 78 cells, MOLT-4 cells, ACHN cells, Raji cells
Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy PSMB8 Ready-To-Use IHC Kit. PSMB8 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

86241-1-RR targets PSMB8 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag6660

Product name: Recombinant human PSMB8 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-272 aa of BC001114

Sequence: MLIGTPTPRDTTPSSWLTSSLLVEAAPLDDTTLPTPVSSGCPGLEPTEFFQSLGGDGERNVQIEMAHGTTTLAFKFQHGVIAAVDSRASAGSYISALRVNKVIEINPYLLGTMSGCAADCQYWERLLAKECRLYYLRNGERISVSAASKLLSNMMCQYRGMGLSMGSMICGWDKKGPGLYYVDEHGTRLSGNMFSTGSGNTYAYGVMDSGYRPNLSPEEAYDLGRRAIAYATHRDSYSGGVVNMYHMKEDGWVKVESTDVSDLLHQYREANQ

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 proteasome (prosome, macropain) subunit, beta type, 8 (large multifunctional peptidase 7)
别名 EC:3.4.25.1, LMP7, Proteasome subunit beta type 8, Proteasome subunit beta type-8, Proteasome subunit beta-5i
计算分子量 30 kDa
观测分子量 23 kDa, 30 kDa
GenBank蛋白编号BC001114
基因名称 PSMB8
Gene ID (NCBI) 5696
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP28062
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IF protocol for PSMB8 antibody 86241-1-RRDownload protocol
WB protocol for PSMB8 antibody 86241-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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