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验证数据展示

  • WB analysis using 30283-1-AP

    WB analysis using 30283-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30283-1-AP (PSMB7 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • WB analysis using 30283-1-AP

    WB analysis using 30283-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30283-1-AP (PSMB7 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon cancer using 30283-1-AP

    IHC staining of human colon cancer using 30283-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 30283-1-AP (PSMB7 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PSMB7 antibody included
  • IHC staining of human colon cancer using 30283-1-AP

    IHC staining of human colon cancer using 30283-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 30283-1-AP (PSMB7 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PSMB7 antibody included

PSMB7 Polyclonal antibody

PSMB7 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 30283-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

Human, mouse, rat

应用

WB, IHC, ELISA

别名

Macropain chain Z, Proteasome subunit beta type 7, Proteasome subunit Z, PSMB7, Z

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHEK-293 cells, MCF-7 cells, HepG2 cells, mouse skeletal muscle tissue, rat skeletal muscle tissue, mouse kidney tissue, rat kidney tissue
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy PSMB7 Ready-To-Use IHC Kit. PSMB7 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

30283-1-AP targets PSMB7 in WB, IHC, ELISA applications and shows reactivity with Human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 Human, mouse, rat
免疫原

CatNo: Ag32201

Product name: Recombinant human PSMB7 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 91-277 aa of BC000509

Sequence: TAADTDMTTQLISSNLELHSLSTGRLPRVVTANRMLKQMLFRYQGYIGAALVLGGVDVTGPHLYSIYPHGSTDKLPYVTMGSGSLAAMAVFEDKFRPDMEEEEAKNLVSEAIAAGIFNDLGSGSNIDLCVISKNKLDFLRPYTVPNKKGTRLGRYRCEKGTTAVLTEKITPLEIEVLEETVQTMDTS

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 proteasome (prosome, macropain) subunit, beta type, 7
别名 Macropain chain Z, Proteasome subunit beta type 7, Proteasome subunit Z, PSMB7, Z
计算分子量29.9 kDa
观测分子量28-30 kDa
GenBank蛋白编号BC000509
基因名称 PSMB7
Gene ID (NCBI) 5695
RRIDAB_3086285
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ99436
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. PSMB7 protein is a member of the proteasome B-type family, also known as the T1B family, and is a 20S core beta subunit in the proteasome. Expression of this catalytic subunit is downregulated by gamma interferon, and proteolytic processing is required to generate a mature subunit. Component of the 20S core proteasome complex are involved in the proteolytic degradation of most intracellular proteins. PSMB7 displays a trypsin-like activity.(PMID:15244466, PMID:27176742, PMID: 8610016)

实验方案

Product Specific Protocols
WB protocol for PSMB7 antibody 30283-1-APDownload protocol
IHC protocol for PSMB7 antibody 30283-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols