验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293 cells, MCF-7 cells, HepG2 cells, mouse skeletal muscle tissue, rat skeletal muscle tissue, mouse kidney tissue, rat kidney tissue |
| Positive IHC detected in | human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
30283-1-AP targets PSMB7 in WB, IHC, IF, ELISA applications and shows reactivity with Human, mouse, rat samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | Human, mouse, rat |
| 文献引用反应性 | human |
| 免疫原 |
CatNo: Ag32201 Product name: Recombinant human PSMB7 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 91-277 aa of BC000509 Sequence: TAADTDMTTQLISSNLELHSLSTGRLPRVVTANRMLKQMLFRYQGYIGAALVLGGVDVTGPHLYSIYPHGSTDKLPYVTMGSGSLAAMAVFEDKFRPDMEEEEAKNLVSEAIAAGIFNDLGSGSNIDLCVISKNKLDFLRPYTVPNKKGTRLGRYRCEKGTTAVLTEKITPLEIEVLEETVQTMDTS 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | proteasome (prosome, macropain) subunit, beta type, 7 |
| 别名 | Macropain chain Z, Proteasome subunit beta type 7, Proteasome subunit Z, PSMB7, Z |
| 计算分子量 | 29.9 kDa |
| 观测分子量 | 28-30 kDa |
| GenBank蛋白编号 | BC000509 |
| 基因名称 | PSMB7 |
| Gene ID (NCBI) | 5695 |
| RRID | AB_3086285 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q99436 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. PSMB7 protein is a member of the proteasome B-type family, also known as the T1B family, and is a 20S core beta subunit in the proteasome. Expression of this catalytic subunit is downregulated by gamma interferon, and proteolytic processing is required to generate a mature subunit. Component of the 20S core proteasome complex are involved in the proteolytic degradation of most intracellular proteins. PSMB7 displays a trypsin-like activity.(PMID:15244466, PMID:27176742, PMID: 8610016)
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for PSMB7 antibody 30283-1-AP | Download protocol |
| WB protocol for PSMB7 antibody 30283-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cell Rep 19S proteasome loss regulates mitotic spindle assembly through a ubiquitin-independent degradation mechanism | ||
Cell Cycle Identification of mitochondrial permeability transition-related gene signatures to predict lung adenocarcinoma survival and drug response.
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