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验证数据展示

  • WB analysis using 67959-1-Ig

    WB analysis using 67959-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67959-1-Ig (PSMB5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • IHC staining of human breast cancer using 67959-1-Ig

    IHC staining of human breast cancer using 67959-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67959-1-Ig (PSMB5 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PSMB5 antibody included
  • IF Staining of HeLa using 67959-1-Ig

    IF Staining of HeLa using 67959-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PSMB5 antibody (67959-1-Ig, Clone: 2E9H1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • WB analysis of HeLa using 67959-1-Ig

    WB analysis of HeLa using 67959-1-Ig

    WB result of PSMB5 antibody (67959-1-Ig; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PSMB5 transfected HeLa cells.

  • FC experiment of HepG2 using 67959-1-Ig

    FC experiment of HepG2 using 67959-1-Ig

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug PSMB5 Monoclonal antibody (67959-1-Ig, Clone:2E9H1) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.25 ug Mouse IgG2a isotype control Mouse McAb (66360-2-Ig, Clone: 11A1B2) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

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  • KD/KO验证

PSMB5 Monoclonal antibody

PSMB5 Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA
Cat No. 67959-1-Ig

产品说明书

CloneNo. 2E9H1

宿主/亚型

Mouse / IgG2a

种属反应性

human

应用

WB, IHC, IF/ICC, FC (Intra), ELISA

别名

2E9H1, EC:3.4.25.1, LMPX, MB1, Proteasome subunit beta type-5

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格价格库存

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经过测试的应用

Positive WB detected inA431 cells, HeLa cells, U2OS cells, HEK-293 cells, Jurkat cells, K-562 cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy PSMB5 Ready-To-Use IHC Kit. PSMB5 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67959-1-Ig targets PSMB5 in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, FC (Intra), ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag5828

Product name: Recombinant human PSMB5 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-263 aa of BC057840

Sequence: MALASVLERPLPVNQRGFFGLGGRADLLDLGPGSLSDGLSLAAPGWGVPEEPGIEMLHGTTTLAFKFRHGVIVAADSRATAGAYIASQTVKKVIEINPYLLGTMAGGAADCSFWERLLARQCRIYELRNKERISVAAASKLLANMVYQYKGMGLSMGTMICGWDKRGPGLYYVDSEGNRISGATFSVGSGSVYAYGVMDRGYSYDLEVEQAYDLARRAIYQATYRDAYSGGAVNLYHVREDGWIRVSSDNVADLHEKYSGSTP

种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 proteasome (prosome, macropain) subunit, beta type, 5
别名 2E9H1, EC:3.4.25.1, LMPX, MB1, Proteasome subunit beta type-5
计算分子量 263 aa, 28 kDa
观测分子量22 kDa
GenBank蛋白编号BC057840
基因名称 PSMB5
Gene ID (NCBI) 5693
RRIDAB_2918710
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP28074
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PSMB5(Proteasome subunit beta type-5) is also named as LMPX, MB1, X and belongs to the peptidase T1B family.The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH.RNAi-mediated silencing of.It has 2 isoforms produced by alternative splicing.

实验方案

Product Specific Protocols
IF protocol for PSMB5 antibody 67959-1-IgDownload protocol
IHC protocol for PSMB5 antibody 67959-1-IgDownload protocol
WB protocol for PSMB5 antibody 67959-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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