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  • Featured Product
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PSMB5 Monoclonal antibody

PSMB5 Monoclonal Antibody for IF/ICC, IHC, WB, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human

Applications

IF/ICC, IHC, WB, ELISA

Conjugate

Unconjugated

CloneNo.

2E9H1

Cat No : 67959-1-Ig

Print datasheet

Synonyms

LMPX, Macropain epsilon chain, MB1, Proteasome chain 6, Proteasome epsilon chain, Proteasome subunit beta type 5, Proteasome subunit MB1, Proteasome subunit X, PSMB5, X

验证数据展示

  • WB analysis using 67959-1-Ig

    WB analysis using 67959-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67959-1-Ig (PSMB5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • IHC staining of human breast cancer using 67959-1-Ig

    IHC staining of human breast cancer using 67959-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67959-1-Ig (PSMB5 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PSMB5 antibody included
  • IF Staining of HeLa using 67959-1-Ig

    IF Staining of HeLa using 67959-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PSMB5 antibody (67959-1-Ig, Clone: 2E9H1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • WB analysis of HeLa using 67959-1-Ig

    WB analysis of HeLa using 67959-1-Ig

    WB result of PSMB5 antibody (67959-1-Ig; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PSMB5 transfected HeLa cells.

经过测试的应用

Positive WB detected inA431 cells, HeLa cells, U2OS cells, HEK-293 cells, Jurkat cells, K-562 cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy PSMB5 Ready-To-Use IHC Kit. PSMB5 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67959-1-Ig targets PSMB5 in WB, IF, IHC, ELISA applications and shows reactivity with Human samples.

Tested Applications IF/ICC, IHC, WB, ELISA
Tested Reactivity Human
Immunogen PSMB5 fusion protein Ag5828 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name proteasome (prosome, macropain) subunit, beta type, 5
Synonyms LMPX, Macropain epsilon chain, MB1, Proteasome chain 6, Proteasome epsilon chain, Proteasome subunit beta type 5, Proteasome subunit MB1, Proteasome subunit X, PSMB5, X
Calculated Molecular Weight 263 aa, 28 kDa
Observed Molecular Weight22 kDa
GenBank Accession NumberBC057840
Gene Symbol PSMB5
Gene ID (NCBI) 5693
RRIDAB_2918710
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP28074
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PSMB5(Proteasome subunit beta type-5) is also named as LMPX, MB1, X and belongs to the peptidase T1B family.The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH.RNAi-mediated silencing of.It has 2 isoforms produced by alternative splicing.

实验方案

Product Specific Protocols
WB protocol for PSMB5 antibody 67959-1-IgDownload protocol
IHC protocol for PSMB5 antibody 67959-1-IgDownload protocol
IF protocol for PSMB5 antibody 67959-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols