验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293 cells, mouse skeletal muscle tissue, human placenta tissue, human skeletal muscle tissue, MCF-7 cells |
| Positive IHC detected in | human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | MCF-7 cells, HepG2 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
11029-1-AP targets PSMB4 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human, mouse, pig, monkey |
| 免疫原 | PSMB4 fusion protein Ag1500 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | proteasome (prosome, macropain) subunit, beta type, 4 |
| 别名 | Proteasome subunit beta type-4, PROS-26, PROS26, HsN3 |
| 计算分子量 | 29 kDa |
| 观测分子量 | 25 kDa |
| GenBank蛋白编号 | BC010088 |
| 基因名称 | PSMB4 |
| Gene ID (NCBI) | 5692 |
| RRID | AB_2172040 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P28070 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
PSMB4(Proteasome subunit beta type-4) is also named as PROS26 and belongs to the peptidase T1B family. The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The human protein PSMB4 is known mainly as an interaction partner for several proteins, suggesting an important function of binding not only to the lid, but also to the 20 S core of the proteasome. PSMB4 might be a major site for proteasome regulation, where signals from the outside might be transduced to the protease activities inside(PMID:15660529 ). The full length 30 kDa protein has a propeptide of 45 amino acid.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for PSMB4 antibody 11029-1-AP | Download protocol |
| IHC protocol for PSMB4 antibody 11029-1-AP | Download protocol |
| IF protocol for PSMB4 antibody 11029-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cancer Res Comparative oncogenomics identifies PSMB4 and SHMT2 as potential cancer driver genes. | ||
J Biol Chem Hepatocyte-specific Wtap deficiency promotes hepatocellular carcinoma by activating GRB2/ERK depending on downregulation of proteasome-related genes | ||
Oncol Rep PSMB4 overexpression enhances the cell growth and viability of breast cancer cells leading to a poor prognosis.
| ||
J Virol PSMB4 Degrades the Porcine Reproductive and Respiratory Syndrome Virus Nsp1α Protein via the Autolysosome Pathway and Induces the Production of Type I Interferon
| ||
JHEP Rep Downregulation of hepatic METTL3 contributes to APAP-induced liver injury in mice |
