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验证数据展示

  • WB analysis using 15983-1-AP

    WB analysis using 15983-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15983-1-AP (PSMB3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver using 15983-1-AP

    IHC staining of human liver using 15983-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 15983-1-AP (PSMB3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PSMB3 antibody included
  • IF Staining of Jurkat using 15983-1-AP

    IF Staining of Jurkat using 15983-1-AP

    Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using PSMB3 antibody (15983-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

  • WB analysis using 15983-1-AP

    WB analysis using 15983-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15983-1-AP (PSMB3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

PSMB3 Polyclonal antibody

PSMB3 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 15983-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, ELISA

别名

Proteasome subunit beta type-3, Proteasome component C10-II, HC10 II

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inJurkat cells, HEK-293T cells, mouse testis tissue, rat testis tissue, HeLa cells, MCF-7 cells
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inJurkat cells
Planning an IHC experiment? We recommend our IHCeasy PSMB3 Ready-To-Use IHC Kit. PSMB3 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

15983-1-AP targets PSMB3 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag8784

Product name: Recombinant human PSMB3 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-205 aa of BC013008

Sequence: MSIMSYNGGAVMAMKGKNCVAIAADRRFGIQAQMVTTDFQKIFPMGDRLYIGLAGLATDVQTVAQRLKFRLNLYELKEGRQIKPYTLMSMVANLLYEKRFGPYYTEPVIAGLDPKTFKPFICSLDLIGCPMVTDDFVVSGTCAEQMYGMCESLWEPNMDPDHLFETISQAMLNAVDRDAVSGMGVIVHIIEKDKITTRTLKARMD

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 proteasome (prosome, macropain) subunit, beta type, 3
别名 Proteasome subunit beta type-3, Proteasome component C10-II, HC10 II
计算分子量 205 aa, 23 kDa
观测分子量 23-27 kDa
GenBank蛋白编号BC013008
基因名称 PSMB3
Gene ID (NCBI) 5691
RRIDAB_2172027
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP49720
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IF protocol for PSMB3 antibody 15983-1-APDownload protocol
IHC protocol for PSMB3 antibody 15983-1-APDownload protocol
WB protocol for PSMB3 antibody 15983-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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