• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 66403-1-Ig (same clone as 66403-1-PBS)

    WB analysis using 66403-1-Ig (same clone as 66403-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66403-1-Ig (PSMB10 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 66403-1-PBS in a different storage buffer formulation.

  • WB analysis of Raji using 66403-1-Ig (same clone as 66403-1-PBS)

    WB analysis of Raji using 66403-1-Ig (same clone as 66403-1-PBS)

    Raji cells were subjected to SDS PAGE followed by western blot with 66403-1-Ig (PSMB10 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66403-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 66403-1-Ig (same clone as 66403-1-PBS)

    IHC staining of human liver cancer using 66403-1-Ig (same clone as 66403-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66403-1-Ig (PSMB10 antibody) at dilution of 1:300 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66403-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PSMB10 antibody included
  • IHC staining of human liver cancer using 66403-1-Ig (same clone as 66403-1-PBS)

    IHC staining of human liver cancer using 66403-1-Ig (same clone as 66403-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66403-1-Ig (PSMB10 antibody) at dilution of 1:300 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66403-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PSMB10 antibody included
  • WB analysis of rat thymus using 66403-1-Ig (same clone as 66403-1-PBS)

    WB analysis of rat thymus using 66403-1-Ig (same clone as 66403-1-PBS)

    rat thymus tissue were subjected to SDS PAGE followed by western blot with 66403-1-Ig (PSMB10 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66403-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse kidney using 66403-1-Ig (same clone as 66403-1-PBS)

    IHC staining of mouse kidney using 66403-1-Ig (same clone as 66403-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 66403-1-Ig (PSMB10 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66403-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PSMB10 antibody included
  • IF Staining of Jurkat using 66403-1-Ig (same clone as 66403-1-PBS)

    IF Staining of Jurkat using 66403-1-Ig (same clone as 66403-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using PSMB10 antibody (66403-1-Ig, Clone: 1G8C1 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 66403-1-PBS in a different storage buffer formulation.

PSMB10 Monoclonal antibody, PBS Only

PSMB10 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 66403-1-PBS

产品说明书

CloneNo. 1G8C1

宿主/亚型

Mouse / IgG2a

种属反应性

human, mouse, rat, pig

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

Multicatalytic endopeptidase complex subunit MECl-1, MECL1, Macropain subunit MECl-1, LMP10, EC:3.4.25.1

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

Planning an IHC experiment? We recommend our IHCeasy PSMB10 Ready-To-Use IHC Kit. PSMB10 primary antibody included.

产品信息

66403-1-PBS targets PSMB10 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat, pig samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat, pig
免疫原 PSMB10 fusion protein Ag8835 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 proteasome (prosome, macropain) subunit, beta type, 10
别名 Multicatalytic endopeptidase complex subunit MECl-1, MECL1, Macropain subunit MECl-1, LMP10, EC:3.4.25.1
计算分子量 273 aa, 29 kDa
观测分子量 28 kDa
GenBank蛋白编号BC017198
基因名称 PSMB10
Gene ID (NCBI) 5699
RRIDAB_2881777
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP40306
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

SMB10(Proteasome subunit beta type-10) is also named as LMP10, MECL1.It belongs to the peptidase T1B family and is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH.