验证数据展示
经过测试的应用
| Positive WB detected in | MCF-7 cells, human brain tissue, HepG2 cells, HeLa cells, NIH/3T3 cells |
| Positive IHC detected in | human liver cancer tissue, human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | U2OS cells |
| Positive FC (Intra) detected in | HeLa cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
11749-1-AP targets PSMB1 in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, FC (Intra), ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 |
CatNo: Ag2345 Product name: Recombinant human PSMB1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-241 aa of BC020807 Sequence: MLSSTAMYSAPGRDLGMEPHRAAGPLQLRFSPYVFNGGTILAIAGEDFAIVASDTRLSEGFSIHTRDSPKCYKLTDKTVIGCSGFHGDCLTLTKIIEARLKMYKHSNNKAMTTGAIAAMLSTILYSRRFFPYYVYNIIGGLDEEGKGAVYSFDPVGSYQRDSFKAGGSASAMLQPLLDNQVGFKNMQNVEHVPLSLDRAMRLVKDVFISAAERDVYTGDALRICIVTKEGIREETVSLRKD 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | proteasome (prosome, macropain) subunit, beta type, 1 |
| 别名 | FLJ25321, HC5, KIAA1838, Macropain subunit C5, PMSB1 |
| 计算分子量 | 241 aa, 26 kDa |
| 观测分子量 | 25 kDa |
| GenBank蛋白编号 | BC020807 |
| 基因名称 | PSMB1 |
| Gene ID (NCBI) | 5689 |
| RRID | AB_2284481 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P20618 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
PSMB1(Proteasome subunit beta type-1) is also named as PSC5 and belongs to the peptidase T1B family. The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The gene encodes a 241 amino acid protein with a 28 amino acid propeptide and two glycosylation sites.
实验方案
| Product Specific Protocols | |
|---|---|
| FC protocol for PSMB1 antibody 11749-1-AP | Download protocol |
| IF protocol for PSMB1 antibody 11749-1-AP | Download protocol |
| IHC protocol for PSMB1 antibody 11749-1-AP | Download protocol |
| WB protocol for PSMB1 antibody 11749-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cell Comprehensive human proteome profiles across a 50-year lifespan reveal aging trajectories and signatures | ||
Nat Neurosci Neuroproteasomes regulate endogenous tau paired helical filament formation in an APOE genotype- and age-dependent manner. | ||
Nat Neurosci Dendritic translation and neuroproteasome-mediated degradation of endogenous tau revealed by STARFISH. | ||
Cell Death Differ Quantitative reactive cysteinome profiling reveals a functional link between ferroptosis and proteasome-mediated degradation | ||
Cancer Lett Combinations of ivermectin with proteasome inhibitors induce synergistic lethality in multiple myeloma | ||
Phytomedicine PTEN status determines therapeutic vulnerability to celastrol in cholangiocarcinoma |
