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PSMA8 Monoclonal antibody

PSMA8 Monoclonal Antibody for IF/ICC, IHC, WB, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, mouse, rat, pig, rabbit

Applications

IF/ICC, IHC, WB, ELISA

Conjugate

Unconjugated

CloneNo.

2G2G3

Cat No : 68123-1-Ig

Print datasheet

Synonyms

PSMA7L, PSMA8

验证数据展示

  • WB analysis using 68123-1-Ig

    WB analysis using 68123-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68123-1-Ig (PSMA8 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis of rat lung using 68123-1-Ig

    WB analysis of rat lung using 68123-1-Ig

    rat lung tissue were subjected to SDS PAGE followed by western blot with 68123-1-Ig (PSMA8 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis of mouse lung using 68123-1-Ig

    WB analysis of mouse lung using 68123-1-Ig

    mouse lung tissue were subjected to SDS PAGE followed by western blot with 68123-1-Ig (PSMA8 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis of HeLa using 68123-1-Ig

    WB analysis of HeLa using 68123-1-Ig

    WB result of PSMA8 antibody (68123-1-Ig; 1:80000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PSMA8 transfected HeLa cells.

  • IHC staining of mouse testis using 68123-1-Ig

    IHC staining of mouse testis using 68123-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 68123-1-Ig (PSMA8 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse testis using 68123-1-Ig

    IHC staining of mouse testis using 68123-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 68123-1-Ig (PSMA8 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HepG2 using 68123-1-Ig

    IF Staining of HepG2 using 68123-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using PSMA8 antibody (68123-1-Ig, Clone: 2G2G3 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

经过测试的应用

Positive WB detected inHCT 116 cells, rat lung tissue, mouse lung tissue, HeLa cells, HuH-7 cells, NCI-H1299 cells, A549 cells, pig lung tissue, rabbit lung tissue
Positive IHC detected inmouse testis tissue, human colon cancer tissue, human lung cancer tissue, mouse liver tissue, rat liver tissue, rat testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)IF : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68123-1-Ig targets PSMA8 in WB, IF, IHC, ELISA applications and shows reactivity with Human, mouse, rat, pig, rabbit samples.

Tested Applications IF/ICC, IHC, WB, ELISA
Tested Reactivity Human, mouse, rat, pig, rabbit
Immunogen PSMA8 fusion protein Ag5138 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name proteasome (prosome, macropain) subunit, alpha type, 8
Synonyms PSMA7L, PSMA8
Calculated Molecular Weight 29 kDa
Observed Molecular Weight28 kDa
GenBank Accession NumberBC042820
Gene Symbol PSMA8
Gene ID (NCBI) 143471
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ8TAA3
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

1

实验方案

Product Specific Protocols
WB protocol for PSMA8 antibody 68123-1-IgDownload protocol
IHC protocol for PSMA8 antibody 68123-1-IgDownload protocol
IF protocol for PSMA8 antibody 68123-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols