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验证数据展示

  • WB analysis using 67695-1-Ig (same clone as 67695-1-PBS)

    WB analysis using 67695-1-Ig (same clone as 67695-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67695-1-Ig (PSMA6 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67695-1-PBS in a different storage buffer formulation.

  • IHC staining of human lung cancer using 67695-1-Ig (same clone as 67695-1-PBS)

    IHC staining of human lung cancer using 67695-1-Ig (same clone as 67695-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67695-1-Ig (PSMA6 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67695-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PSMA6 antibody included
  • IF Staining of human lung cancer using 67695-1-Ig (same clone as 67695-1-PBS)

    IF Staining of human lung cancer using 67695-1-Ig (same clone as 67695-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human lung cancer tissue using PSMA6 antibody (67695-1-Ig, Clone: 1D4B2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67695-1-PBS in a different storage buffer formulation.

  • FC experiment of HepG2 using 67695-1-Ig (same clone as 67695-1-PBS)

    FC experiment of HepG2 using 67695-1-Ig (same clone as 67695-1-PBS)

    1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human PSMA6 (67695-1-Ig, Clone:1D4B2) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 67695-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 67695-1-Ig (same clone as 67695-1-PBS)

    IF Staining of HeLa using 67695-1-Ig (same clone as 67695-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PSMA6 antibody (67695-1-Ig, Clone: 1D4B2 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67695-1-PBS in a different storage buffer formulation.

PSMA6 Monoclonal antibody, PBS Only

PSMA6 Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, FC (Intra), ELISA
Cat No. 67695-1-PBS

产品说明书

CloneNo. 1D4B2

宿主/亚型

Mouse / IgG2b

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, IF-P, FC (Intra), ELISA

别名

PROS-27, PROS27, PROS 27, p27K, IOTA

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy PSMA6 Ready-To-Use IHC Kit. PSMA6 primary antibody included.

产品信息

67695-1-PBS targets PSMA6 in WB, IHC, IF/ICC, IF-P, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IF-P, FC (Intra), ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 PSMA6 fusion protein Ag21761 种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 proteasome (prosome, macropain) subunit, alpha type, 6
别名 PROS-27, PROS27, PROS 27, p27K, IOTA
计算分子量 246 aa, 27 kDa
观测分子量30 kDa
GenBank蛋白编号BC023659
基因名称 PSMA6
Gene ID (NCBI) 5687
RRIDAB_2882887
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP60900
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

PSMA6(Proteasome subunit alpha type-6) is also named as PROS27 and belongs to the peptidase T1A family.The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH.It also has an ATP-dependent proteolytic activity.