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PSMA5 Polyclonal antibody

PSMA5 Polyclonal Antibody for IF/ICC, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse

Applications

IF/ICC, IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 30580-1-AP

Print datasheet

Synonyms

Macropain zeta chain, Proteasome zeta chain, PSC5, PSMA5, ZETA

验证数据展示

  • WB analysis using 30580-1-AP

    WB analysis using 30580-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30580-1-AP (PSMA5 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse testis using 30580-1-AP

    IHC staining of mouse testis using 30580-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 30580-1-AP (PSMA5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PSMA5 antibody included
  • IF Staining of HeLa using 30580-1-AP

    IF Staining of HeLa using 30580-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PSMA5 antibody (30580-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

经过测试的应用

Positive WB detected inHeLa cells, HCT 116 cells, HepG2 cells
Positive IHC detected inmouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy PSMA5 Ready-To-Use IHC Kit. PSMA5 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

30580-1-AP targets PSMA5 in WB, IF, IHC, ELISA applications and shows reactivity with Human, mouse samples.

Tested Applications IF/ICC, IHC, WB, ELISA
Tested Reactivity Human, mouse
Immunogen PSMA5 fusion protein Ag30703 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name proteasome (prosome, macropain) subunit, alpha type, 5
Synonyms Macropain zeta chain, Proteasome zeta chain, PSC5, PSMA5, ZETA
Calculated Molecular Weight 26 kDa
Observed Molecular Weight28 kDa
GenBank Accession NumberNM_002790
Gene Symbol PSMA5
Gene ID (NCBI) 5686
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP28066
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Proteasome subunit alpha type-5(PSMA5) is a component of the 20S core proteasome complex involved in the proteolytic degradation of most intracellular proteins. This complex plays numerous essential roles within the cell by associating with different regulatory particles. It binds to two 19S regulatory particles(RP) to form the 26S proteasome, an ATP-dependent multisubunit protease that degrades polyubiquitinated proteins into small peptides.(PMID: 26661102)

实验方案

Product Specific Protocols
WB protocol for PSMA5 antibody 30580-1-APDownload protocol
IHC protocol for PSMA5 antibody 30580-1-APDownload protocol
IF protocol for PSMA5 antibody 30580-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols