• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 30580-1-AP

    WB analysis using 30580-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30580-1-AP (PSMA5 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse testis using 30580-1-AP

    IHC staining of mouse testis using 30580-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 30580-1-AP (PSMA5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PSMA5 antibody included
  • IF Staining of HeLa using 30580-1-AP

    IF Staining of HeLa using 30580-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PSMA5 antibody (30580-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

PSMA5 Polyclonal antibody

PSMA5 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 30580-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, ELISA

别名

ZETA, PSC5, Proteasome subunit alpha type-5

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive WB detected inHeLa cells, HCT 116 cells, HepG2 cells
Positive IHC detected inmouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy PSMA5 Ready-To-Use IHC Kit. PSMA5 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

30580-1-AP targets PSMA5 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse
免疫原 PSMA5 fusion protein Ag30703 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 proteasome (prosome, macropain) subunit, alpha type, 5
别名 ZETA, PSC5, Proteasome subunit alpha type-5
计算分子量 26 kDa
观测分子量28 kDa
GenBank蛋白编号NM_002790
基因名称 PSMA5
Gene ID (NCBI) 5686
RRIDAB_3086368
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP28066
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Proteasome subunit alpha type-5(PSMA5) is a component of the 20S core proteasome complex involved in the proteolytic degradation of most intracellular proteins. This complex plays numerous essential roles within the cell by associating with different regulatory particles. It binds to two 19S regulatory particles(RP) to form the 26S proteasome, an ATP-dependent multisubunit protease that degrades polyubiquitinated proteins into small peptides.(PMID: 26661102)

实验方案

Product Specific Protocols
WB protocol for PSMA5 antibody 30580-1-APDownload protocol
IHC protocol for PSMA5 antibody 30580-1-APDownload protocol
IF protocol for PSMA5 antibody 30580-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols