• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

PSKH2 Polyclonal antibody

PSKH2 Polyclonal Antibody for IF/ICC, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, IHC, IP, WB,ELISA

Conjugate

Unconjugated

Cat No : 18813-1-AP

Print datasheet

Synonyms

protein serine kinase H2, PSK H2, PSKH2

验证数据展示

  • WB analysis of HepG2 using 18813-1-AP

    WB analysis of HepG2 using 18813-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 18813-1-AP (PSKH2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of human kidney using 18813-1-AP

    IHC staining of human kidney using 18813-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 18813-1-AP (PSKH2 antibody) at dilution of 1:100 (under 10x lens).

  • IHC staining of human kidney using 18813-1-AP

    IHC staining of human kidney using 18813-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 18813-1-AP (PSKH2 antibody) at dilution of 1:100 (under 40x lens).

  • IP experiment of HepG2 using 18813-1-AP

    IP experiment of HepG2 using 18813-1-AP

    IP result of anti-PSKH2 (IP:18813-1-AP, 4ug; Detection:18813-1-AP 1:500) with HepG2 cells lysate 3400ug.

  • IF Staining of HepG2 using 18813-1-AP

    IF Staining of HepG2 using 18813-1-AP

    Immunofluorescent analysis of (10% Formaldehyde) fixed HepG2 cells using 18813-1-AP (PSKH2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inHepG2 cells, human brain tissue, human heart tissue, human testis tissue
Positive IP detected inHepG2 cells
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

18813-1-AP targets PSKH2 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications IF/ICC, IHC, IP, WB,ELISA
Tested Reactivity human
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name protein serine kinase H2
Synonyms protein serine kinase H2, PSK H2, PSKH2
Calculated Molecular Weight 43 kDa
Observed Molecular Weight 35 kDa
GenBank Accession NumberBC126182
Gene Symbol PSKH2
Gene ID (NCBI) 85481
RRIDAB_2171244
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96QS6
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PSKH2 belongs to the protein kinase superfamily and CAMK Ser/Thr protein kinase family. PSKH2 catalytic the reaction: ATP + a protein = ADP + a phosphoprotein.

实验方案

Product Specific Protocols
WB protocol for PSKH2 antibody 18813-1-APDownload protocol
IHC protocol for PSKH2 antibody 18813-1-APDownload protocol
IF protocol for PSKH2 antibody 18813-1-APDownload protocol
IP protocol for PSKH2 antibody 18813-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols