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验证数据展示

  • WB analysis using 29434-1-AP

    WB analysis using 29434-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29434-1-AP (PSIP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 29434-1-AP

    IF Staining of HepG2 using 29434-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using PSIP1 antibody (29434-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

PSIP1 Polyclonal antibody

PSIP1 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 29434-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

Human, mouse

应用

WB, IF/ICC, ELISA

别名

CLL associated antigen KW 7, DFS 70, DFS70, LEDGF, p52, p75, PAIP, PSIP1, PSIP2

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHeLa cells, HepG2 cells, MCF-7 cells, Jurkat cells, NIH/3T3 cells
Positive IF/ICC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy PSIP1 Ready-To-Use IHC Kit. PSIP1 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

29434-1-AP targets PSIP1 in WB, IF/ICC, ELISA applications and shows reactivity with Human, mouse samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 Human, mouse
免疫原

CatNo: Ag30640

Product name: Recombinant human PSIP1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 104-325 aa of NM_021144

Sequence: ASSDVEVEEKETSVSKEDTDHEEKASNEDVTKAVDITTPKAARRGRKRKAEKQVETEEAGVVTTATASVNLKVSPKRGRPAATEVKIPKPRGRPKMVKQPCPSESDIITEEDKSKKKGQEEKQPKKQPKKDEEGQKEEDKPRKEPDKKEGKKEVESKRKNLAKTGVTSTSDSEEEGDDQEGEKKRKGGRNFQTAHRRNMLKGQHEKEAADRKRKQEEQMETE

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 PC4 and SFRS1 interacting protein 1
别名 CLL associated antigen KW 7, DFS 70, DFS70, LEDGF, p52, p75, PAIP, PSIP1, PSIP2
计算分子量 60 kDa
观测分子量75 kDa
GenBank蛋白编号NM_021144
基因名称 PSIP1
Gene ID (NCBI) 11168
RRIDAB_3086132
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDO75475
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PSIP1 enables DNA-binding transcription factor binding activity, chromatin binding activity and transcription coactivator activity. It is involved in mRNA 5'-splice site recognition and positive regulation of transcription by RNA polymerase II.

实验方案

Product Specific Protocols
IF protocol for PSIP1 antibody 29434-1-APDownload protocol
WB protocol for PSIP1 antibody 29434-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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