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验证数据展示

  • WB analysis using 15375-1-AP

    WB analysis using 15375-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15375-1-AP (PSG3 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • IHC staining of human placenta using 15375-1-AP

    IHC staining of human placenta using 15375-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 15375-1-AP (PSG3 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

PSG3 Polyclonal antibody

PSG3 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 15375-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, ELISA

别名

Carcinoembryonic antigen SG5, Pregnancy-specific beta-1-glycoprotein 3, Pregnancy-specific glycoprotein 3, PS beta G 3, PS-beta-G-3

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inhuman placenta tissue
Positive IHC detected inhuman placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:16000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

15375-1-AP targets PSG3 in WB, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag1148

Product name: Recombinant human PSG3 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 129-428 aa of BC005924

Sequence: DGTRGETGHFTFTLYLETPKPSISSSNLYPREDMEAVSLTCDPETPDASYLWWMNGQSLPMTHSLQLSKNKRTLFLFGVTKYTAGPYECEIRNPVSASRSDPVTLNLLPKLPKPYITINNLNPRENKDVLAFTCEPKSENYTYIWWLNGQSLPVSPRVKRPIENRILILPSVTRNETGPYQCEIQDRYGGIRSYPVTLNVLYGPDLPRIYPSFTYYHSGENLYLSCFADSNPPAEYSWTINGKFQLSGQKLFIPQITTKHSGLYACSVRNSATGMESSKSMTVEVSAPSGTGHLPGLNPL

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 pregnancy specific beta-1-glycoprotein 3
别名 Carcinoembryonic antigen SG5, Pregnancy-specific beta-1-glycoprotein 3, Pregnancy-specific glycoprotein 3, PS beta G 3, PS-beta-G-3
计算分子量 48 kDa
观测分子量48 kDa, 64 kDa
GenBank蛋白编号BC005924
基因名称 PSG3
Gene ID (NCBI) 5671
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ16557
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The human pregnancy-specific glycoproteins (PSGs) are a group of molecules that are mainly produced by the placental syncytiotrophoblasts during pregnancy (PMID: 1644821). The calculated molecular weight of PSG3 is 48 kDa. The 64 kDa band is its glycosylated protein form (PMID: 7851896).

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