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PSG11 Polyclonal antibody

PSG11 Polyclonal Antibody for IF/ICC, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IF/ICC, IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 16352-1-AP

Print datasheet

Synonyms

PS beta G 11, PS beta G 13, PSBG 11, PSBG 13, PSG11, PSG13, PSG14

验证数据展示

  • WB analysis of mouse uterus using 16352-1-AP

    WB analysis of mouse uterus using 16352-1-AP

    mouse uterus tissue were subjected to SDS PAGE followed by western blot with 16352-1-AP (PSG11 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of human brain using 16352-1-AP

    IHC staining of human brain using 16352-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 16352-1-AP (PSG11 antibody) at dilution of 1:100 (under 10x lens).

  • IHC staining of human brain using 16352-1-AP

    IHC staining of human brain using 16352-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 16352-1-AP (PSG11 antibody) at dilution of 1:100 (under 40x lens).

  • IF Staining of HeLa using 16352-1-AP

    IF Staining of HeLa using 16352-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 16352-1-AP (PSG11 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inmouse uterus tissue, HeLa cells
Positive IHC detected inhuman brain tissue, human kidney tissue, human liver tissue, human lung tissue, human placenta tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

16352-1-AP targets PSG11 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications IF/ICC, IHC, WB, ELISA
Tested Reactivity human, mouse
Immunogen PSG11 fusion protein Ag9562 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name pregnancy specific beta-1-glycoprotein 11
Synonyms PS beta G 11, PS beta G 13, PSBG 11, PSBG 13, PSG11, PSG13, PSG14
Calculated Molecular Weight 219 aa, 24 kDa
Observed Molecular Weight 24 kDa
GenBank Accession NumberBC020711
Gene Symbol PSG11
Gene ID (NCBI) 5680
RRIDAB_2171065
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UQ72
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PSG11, also named as PSG13, PSG14, PSBG-11 and PSBG-13, belongs to the immunoglobulin superfamily and CEA family. PSBG are produced in high quantity during pregnancy. PSG11 and other PSGs induce dose-dependent secretion of anti-inflammatory cytokines by human monocytes. (PMID:11327547)

实验方案

Product Specific Protocols
WB protocol for PSG11 antibody 16352-1-APDownload protocol
IHC protocol for PSG11 antibody 16352-1-APDownload protocol
IF protocol for PSG11 antibody 16352-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols