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验证数据展示

  • WB analysis of mouse uterus using 16352-1-AP

    WB analysis of mouse uterus using 16352-1-AP

    mouse uterus tissue were subjected to SDS PAGE followed by western blot with 16352-1-AP (PSG11 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of human brain using 16352-1-AP

    IHC staining of human brain using 16352-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 16352-1-AP (PSG11 antibody) at dilution of 1:100 (under 10x lens).

  • IHC staining of human brain using 16352-1-AP

    IHC staining of human brain using 16352-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 16352-1-AP (PSG11 antibody) at dilution of 1:100 (under 40x lens).

  • IF Staining of HeLa using 16352-1-AP

    IF Staining of HeLa using 16352-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PSG11 antibody (16352-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

PSG11 Polyclonal antibody

PSG11 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 16352-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, ELISA

别名

Pregnancy-specific beta-1-glycoprotein 11, Pregnancy-specific beta-1-glycoprotein 13, Pregnancy-specific glycoprotein 11, Pregnancy-specific glycoprotein 13, PS beta G 11

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inmouse uterus tissue, HeLa cells
Positive IHC detected inhuman brain tissue, human kidney tissue, human liver tissue, human lung tissue, human placenta tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

16352-1-AP targets PSG11 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse
免疫原

CatNo: Ag9562

Product name: Recombinant human PSG11 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-219 aa of BC020711

Sequence: MHAAEIMGPLSAPPCTEHIKWKGLLLTVETPKPSISSSNLNPREAMETVILTCNPETPDASYLWWMNGQSLPMTHRMQLSETNRTLFLFGVTKYTAGPYECEIWNSGSASRSDPVTLNLLHGPDLPRIFPSVTSYYSGENLDLSCFADSNPPAQYSWTINGKFQLSGQKLFIPQITPKHNGLYACSARNSATGEESSTSLTIRVIAPPGLGTFAFNNPT

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 pregnancy specific beta-1-glycoprotein 11
别名 Pregnancy-specific beta-1-glycoprotein 11, Pregnancy-specific beta-1-glycoprotein 13, Pregnancy-specific glycoprotein 11, Pregnancy-specific glycoprotein 13, PS beta G 11
计算分子量 219 aa, 24 kDa
观测分子量 24 kDa
GenBank蛋白编号BC020711
基因名称 PSG11
Gene ID (NCBI) 5680
RRIDAB_2171065
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9UQ72
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PSG11, also named as PSG13, PSG14, PSBG-11 and PSBG-13, belongs to the immunoglobulin superfamily and CEA family. PSBG are produced in high quantity during pregnancy. PSG11 and other PSGs induce dose-dependent secretion of anti-inflammatory cytokines by human monocytes. (PMID:11327547)

实验方案

Product Specific Protocols
WB protocol for PSG11 antibody 16352-1-APDownload protocol
IHC protocol for PSG11 antibody 16352-1-APDownload protocol
IF protocol for PSG11 antibody 16352-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
WB

Front Genet

Telomeric Double Strand Breaks in G1 Human Cells Facilitate Formation of 5' C-Rich Overhangs and Recruitment of TERRA.

Authors - Christopher B Nelson
View Article