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PSENEN Polyclonal antibody

PSENEN Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 16144-1-AP

Print datasheet

Synonyms

Gamma secretase subunit PEN 2, MDS033, MSTP064, PEN 2, PEN2, Presenilin enhancer protein 2, PSENEN

验证数据展示

  • WB analysis using 16144-1-AP

    WB analysis using 16144-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16144-1-AP (PSENEN antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse brain using 16144-1-AP

    IHC staining of mouse brain using 16144-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 16144-1-AP (PSENEN Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse brain using 16144-1-AP

    IHC staining of mouse brain using 16144-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 16144-1-AP (PSENEN Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inA549 cells, Neuro-2a cells, mouse lung tissue, rat spleen tissue
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

16144-1-AP targets PSENEN in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IHC, WB, ELISA
Tested Reactivity human, mouse, rat
Immunogen PSENEN fusion protein Ag9243 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name presenilin enhancer 2 homolog (C. elegans)
Synonyms Gamma secretase subunit PEN 2, MDS033, MSTP064, PEN 2, PEN2, Presenilin enhancer protein 2, PSENEN
Calculated Molecular Weight 101 aa, 12 kDa
Observed Molecular Weight12 kDa
GenBank Accession NumberBC009575
Gene Symbol PSENEN
Gene ID (NCBI) 55851
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9NZ42
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PSENEN, also named PEN2, is a component of the gamma-secretase complex, which also includes presenilin (PSEN1) and nicastrin (NCSTN). The gamma-secretase complex is required for the intramembrane proteolysis of a number of membrane proteins, including the amyloid-beta precursor protein (APP) and Notch. PSENEN is required for Notch pathway signaling, and for the activity and accumulation of gamma-secretase. Mutations resulting in haploinsufficiency for this gene cause familial acne inversa-2 (ACNINV2).

实验方案

Product Specific Protocols
WB protocol for PSENEN antibody 16144-1-APDownload protocol
IHC protocol for PSENEN antibody 16144-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols