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验证数据展示

  • WB analysis using 16144-1-AP

    WB analysis using 16144-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16144-1-AP (PSENEN antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse brain using 16144-1-AP

    IHC staining of mouse brain using 16144-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 16144-1-AP (PSENEN Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse brain using 16144-1-AP

    IHC staining of mouse brain using 16144-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 16144-1-AP (PSENEN Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

PSENEN Polyclonal antibody

PSENEN Polyclonal Antibody for WB, IHC, ELISA
Cat No. 16144-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

Presenilin enhancer protein 2, PEN2, PEN 2, Gamma-secretase subunit PEN-2, Gamma secretase subunit PEN 2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inA549 cells, Neuro-2a cells, mouse lung tissue, rat spleen tissue
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

16144-1-AP targets PSENEN in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag9243

Product name: Recombinant human PSENEN protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-101 aa of BC009575

Sequence: MNLERVSNEEKLNLCRKYYLGGFAFLPFLWLVNIFWFFREAFLVPAYTEQSQIKGYVWRSAVGFLFWVIVLTSWITIFQIYRPRWGALGDYLSFTIPLGTP

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 presenilin enhancer 2 homolog (C. elegans)
别名 Presenilin enhancer protein 2, PEN2, PEN 2, Gamma-secretase subunit PEN-2, Gamma secretase subunit PEN 2
计算分子量 101 aa, 12 kDa
观测分子量12 kDa
GenBank蛋白编号BC009575
基因名称 PSENEN
Gene ID (NCBI) 55851
RRIDAB_3669231
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9NZ42
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PSENEN, also named PEN2, is a component of the gamma-secretase complex, which also includes presenilin (PSEN1) and nicastrin (NCSTN). The gamma-secretase complex is required for the intramembrane proteolysis of a number of membrane proteins, including the amyloid-beta precursor protein (APP) and Notch. PSENEN is required for Notch pathway signaling, and for the activity and accumulation of gamma-secretase. Mutations resulting in haploinsufficiency for this gene cause familial acne inversa-2 (ACNINV2).

实验方案

Product Specific Protocols
WB protocol for PSENEN antibody 16144-1-APDownload protocol
IHC protocol for PSENEN antibody 16144-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols