验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, HEK-293 cells, Jurkat cells, K-562 cells, NIH/3T3 cells, pig brain tissue, rabbit brain tissue, rat brain tissue, mouse brain tissue |
| Positive IHC detected in | human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HCT 116 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| KD/KO | See 1 publications below |
| WB | See 3 publications below |
| IHC | See 2 publications below |
产品信息
67619-1-Ig targets PSAT1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, rat, pig samples.
| 经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB, IHC |
| 经测试反应性 | human, rat, pig |
| 文献引用反应性 | human |
| 免疫原 |
CatNo: Ag14076 Product name: Recombinant human PSAT1 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-370 aa of BC001618 Sequence: MDAPRQVVNFGPGPAKLPHSVLLEIQKELLDYKGVGISVLEMSHRSSDFAKIINNTENLVRELLAVPDNYKVIFLQGGGCGQFSAVPLNLIGLKAGRCADYVVTGAWSAKAAEEAKKFGTINIVHPKLGSYTKIPDPSTWNLNPDASYVYYCANETVHGVEFDFIPDVKGAVLVCDMSSNFLSKPVDVSKFGVIFAGAQKNVGSAGVTVVIVRDDLLGFALRECPSVLEYKVQAGNSSLYNTPPCFSIYVMGLVLEWIKNNGGAAAMEKLSSIKSQTIYEIIDNSQGFYVCPVEPQNRSKMNIPFRIGNAKGDDALEKRFLDKALELNMLSLKGHRSVGGIRASLYNAVTIEDVQKLAAFMKKFLEMHQL 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | phosphoserine aminotransferase 1 |
| 别名 | 1B2C9, EC:2.6.1.52, EPIP, Phosphoserine aminotransferase, PSA |
| 计算分子量 | 370 aa, 40 kDa |
| 观测分子量 | 37-40 kDa |
| GenBank蛋白编号 | BC001618 |
| 基因名称 | PSAT1 |
| Gene ID (NCBI) | 29968 |
| RRID | AB_2882821 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| UNIPROT ID | Q9Y617 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
PSAT1, also named as PSA and PSAT, belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family and SerC subfamily. It catalyzes the reversible conversion of 3-phosphohydroxypyruvate to phosphoserine and of 3-hydroxy-2-oxo-4-phosphonooxybutanoate to phosphohydroxythreonine. PSAT1 represents a new interesting target for CRC therapy. (PMID:18221502) It may be implicated in altered serine metabolism and schizophrenia spectrum conditions. (PMID:20955740 )
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for PSAT1 antibody 67619-1-Ig | Download protocol |
| IHC protocol for PSAT1 antibody 67619-1-Ig | Download protocol |
| WB protocol for PSAT1 antibody 67619-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Thorac Cancer Exosomal ERBB2IP contributes to tumor growth via elevating PSAT1 expression in non-small cell lung carcinoma | ||
Cancer Biother Radiopharm Prognostic Signature in Osteosarcoma Based on Amino Acid Metabolism-Associated Genes | ||
Exp Mol Med The m6A writer RBM15 drives the growth of triple-negative breast cancer cells through the stimulation of serine and glycine metabolism | ||
Cancer Metab Serine starvation suppresses the progression of esophageal cancer by regulating the synthesis of purine nucleotides and NADPH
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