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PSAT1 Monoclonal antibody

PSAT1 Monoclonal Antibody for WB, IF/ICC, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat, pig and More (1)

Applications

WB, IF/ICC, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

1B2C9

Cat No : 67619-1-Ig

Print datasheet

Synonyms

EPIP, Phosphoserine aminotransferase, PSA, PSAT, PSAT1

验证数据展示

  • WB analysis using 67619-1-Ig

    WB analysis using 67619-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67619-1-Ig (PSAT1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • IHC staining of human breast cancer using 67619-1-Ig

    IHC staining of human breast cancer using 67619-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67619-1-Ig (PSAT1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HCT 116 using 67619-1-Ig

    IF Staining of HCT 116 using 67619-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HCT 116 cells using PSAT1 antibody (67619-1-Ig, Clone: 1B2C9 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

经过测试的应用

Positive WB detected inHeLa cells, HEK-293 cells, Jurkat cells, K-562 cells, NIH/3T3 cells, pig brain tissue, rabbit brain tissue, rat brain tissue, mouse brain tissue
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHCT 116 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)IF : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67619-1-Ig targets PSAT1 in WB, IF, IHC, ELISA applications and shows reactivity with Human, mouse, rat, pig samples.

Tested Applications WB, IF/ICC, IHC, ELISA
Cited ApplicationsWB, IHC
Tested Reactivity Human, mouse, rat, pig
Cited Reactivityhuman
Immunogen PSAT1 fusion protein Ag14076 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name phosphoserine aminotransferase 1
Synonyms EPIP, Phosphoserine aminotransferase, PSA, PSAT, PSAT1
Calculated Molecular Weight 370 aa, 40 kDa
Observed Molecular Weight 37-40 kDa
GenBank Accession NumberBC001618
Gene Symbol PSAT1
Gene ID (NCBI) 29968
RRIDAB_2882821
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ9Y617
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PSAT1, also named as PSA and PSAT, belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family and SerC subfamily. It catalyzes the reversible conversion of 3-phosphohydroxypyruvate to phosphoserine and of 3-hydroxy-2-oxo-4-phosphonooxybutanoate to phosphohydroxythreonine. PSAT1 represents a new interesting target for CRC therapy. (PMID:18221502) It may be implicated in altered serine metabolism and schizophrenia spectrum conditions. (PMID:20955740 )

实验方案

Product Specific Protocols
WB protocol for PSAT1 antibody 67619-1-IgDownload protocol
IHC protocol for PSAT1 antibody 67619-1-IgDownload protocol
IF protocol for PSAT1 antibody 67619-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,IHC

Thorac Cancer

Exosomal ERBB2IP contributes to tumor growth via elevating PSAT1 expression in non-small cell lung carcinoma

Authors - Xijuan Peng
View Article