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验证数据展示

  • WB analysis of multi-cells/tissue using 55128-1-AP

    WB analysis of multi-cells/tissue using 55128-1-AP

    WB result of 55128-1-AP.

  • WB analysis of human testis using 55128-1-AP

    WB analysis of human testis using 55128-1-AP

    human testis tissue were subjected to SDS PAGE followed by western blot with 55128-1-AP (PSAPL1 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

  • IHC staining of human stomach cancer using 55128-1-AP

    IHC staining of human stomach cancer using 55128-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 55128-1-AP (PSAPL1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human stomach cancer using 55128-1-AP

    IHC staining of human stomach cancer using 55128-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 55128-1-AP (PSAPL1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HepG2 using 55128-1-AP

    IF Staining of HepG2 using 55128-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using PSAPL1 antibody (55128-1-AP) at dilution of 1:200 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4).

PSAPL1 Polyclonal antibody

PSAPL1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 55128-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, ELISA

别名

Saposin B-Val-like, Saposin B-like, Saposin A-like, PSAP L1, prosaposin like 1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inhuman testis tissue, multi-cells/tissue
Positive IHC detected inhuman stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

55128-1-AP targets PSAPL1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 prosaposin-like 1
别名 Saposin B-Val-like, Saposin B-like, Saposin A-like, PSAP L1, prosaposin like 1
计算分子量 57 kDa
观测分子量 57-63 kDa
GenBank蛋白编号NM_001085382
基因名称 PSAPL1
Gene ID (NCBI) 768239
RRIDAB_10888638
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ6NUJ1
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PSAPL1 is a protein like PSAP. It can be cleaved to be 5 chains: Saposin A-like, Saposin B-Val-like, Saposin B-like, Saposin C-like and Saposin D-like. PSAPL1 may activate the lysosomal degradation of sphingolipids. The antibody is specific to PSAPL1.

实验方案

Product Specific Protocols
WB protocol for PSAPL1 antibody 55128-1-APDownload protocol
IHC protocol for PSAPL1 antibody 55128-1-APDownload protocol
IF protocol for PSAPL1 antibody 55128-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols