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验证数据展示

  • IHC staining of human ovary tumor using 11986-1-AP

    IHC staining of human ovary tumor using 11986-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor using 11986-1-AP (PRTFDC1 antibody) at dilution of 1:50 (under 10x lens).

  • WB analysis of fetal human brain using 11986-1-AP

    WB analysis of fetal human brain using 11986-1-AP

    fetal human brain tissue were subjected to SDS PAGE followed by western blot with 11986-1-AP (PRTFDC1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IP experiment of mouse brain using 11986-1-AP

    IP experiment of mouse brain using 11986-1-AP

    IP result of anti-PRTFDC1 (IP:11986-1-AP, 4ug; Detection:11986-1-AP 1:600) with mouse brain tissue lysate 4000ug.

  • IF Staining of HeLa using 11986-1-AP

    IF Staining of HeLa using 11986-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 11986-1-AP (PRTFDC1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

PRTFDC1 Polyclonal antibody

PRTFDC1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 11986-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, IP, ELISA

别名

Phosphoribosyltransferase domain-containing protein 1, HHGP

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected infetal human brain tissue, human brain tissue, Y79 cells
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman ovary tumor tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

11986-1-AP targets PRTFDC1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, IP, ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse
文献引用反应性mouse
免疫原 PRTFDC1 fusion protein Ag2614 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 phosphoribosyl transferase domain containing 1
别名 Phosphoribosyltransferase domain-containing protein 1, HHGP
计算分子量 225 aa, 26 kDa
观测分子量 26 kDa
GenBank蛋白编号BC008662
基因名称 PRTFDC1
Gene ID (NCBI) 56952
RRIDAB_2284509
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9NRG1
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for PRTFDC1 antibody 11986-1-APDownload protocol
IHC protocol for PRTFDC1 antibody 11986-1-APDownload protocol
IF protocol for PRTFDC1 antibody 11986-1-APDownload protocol
IP protocol for PRTFDC1 antibody 11986-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

PLoS One

PRTFDC1 is a genetic modifier of HPRT-deficiency in the mouse.

Authors - Keebaugh Alaine C AC
View Article