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PRTFDC1 Polyclonal antibody

PRTFDC1 Polyclonal Antibody for IF/ICC, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IF/ICC, IHC, IP, WB,ELISA

Conjugate

Unconjugated

Cat No : 11986-1-AP

Print datasheet

Synonyms

HHGP, PRTFDC1

验证数据展示

  • IHC staining of human ovary tumor using 11986-1-AP

    IHC staining of human ovary tumor using 11986-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor using 11986-1-AP (PRTFDC1 antibody) at dilution of 1:50 (under 10x lens).

  • WB analysis of fetal human brain using 11986-1-AP

    WB analysis of fetal human brain using 11986-1-AP

    fetal human brain tissue were subjected to SDS PAGE followed by western blot with 11986-1-AP (PRTFDC1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IP experiment of mouse brain using 11986-1-AP

    IP experiment of mouse brain using 11986-1-AP

    IP result of anti-PRTFDC1 (IP:11986-1-AP, 4ug; Detection:11986-1-AP 1:600) with mouse brain tissue lysate 4000ug.

  • IF Staining of HeLa using 11986-1-AP

    IF Staining of HeLa using 11986-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 11986-1-AP (PRTFDC1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected infetal human brain tissue, human brain tissue, Y79 cells
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman ovary tumor tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

11986-1-AP targets PRTFDC1 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications IF/ICC, IHC, IP, WB,ELISA
Cited ApplicationsWB
Tested Reactivity human, mouse
Cited Reactivitymouse
Immunogen PRTFDC1 fusion protein Ag2614 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name phosphoribosyl transferase domain containing 1
Synonyms HHGP, PRTFDC1
Calculated Molecular Weight 225 aa, 26 kDa
Observed Molecular Weight 26 kDa
GenBank Accession NumberBC008662
Gene Symbol PRTFDC1
Gene ID (NCBI) 56952
RRIDAB_2284509
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9NRG1
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for PRTFDC1 antibody 11986-1-APDownload protocol
IHC protocol for PRTFDC1 antibody 11986-1-APDownload protocol
IF protocol for PRTFDC1 antibody 11986-1-APDownload protocol
IP protocol for PRTFDC1 antibody 11986-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

PLoS One

PRTFDC1 is a genetic modifier of HPRT-deficiency in the mouse.

Authors - Keebaugh Alaine C AC
View Article