验证数据展示
经过测试的应用
| Positive WB detected in | mouse brain tissue, mouse hippocampal tissue |
| Positive IF/ICC detected in | transfected cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
17261-1-AP targets SynDIG4/PRRT1 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 | SynDIG4/PRRT1 fusion protein Ag10958 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | proline-rich transmembrane protein 1 |
| 别名 | PRRT1, Proline-rich transmembrane protein 1, NG5, DSPD1, Dispanin subfamily D member 1 |
| 计算分子量 | 225aa,23 kDa; 306aa,31 kDa |
| 观测分子量 | 35-40 kDa |
| GenBank蛋白编号 | BC013201 |
| 基因名称 | SynDIG4 |
| Gene ID (NCBI) | 80863 |
| RRID | AB_2878371 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q99946 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
SynDIG4 [also known as Prrt1 (proline-rich transmembrane protein 1)] has recently been identified as a component of AMPAR complexes. SynDIG4 is especially prominent in the hippocampus and particularly within CA1.The function of PRRT1 has not been widely studied, and is yet to be fully elucidated.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for SynDIG4/PRRT1 antibody 17261-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Front Synaptic Neurosci Interaction and Subcellular Association of PRRT1/SynDIG4 With AMPA Receptors. | ||
Mol Cell Neurosci PRRT1 regulates basal and plasticity-induced AMPA receptor trafficking. | ||
Zoolog Sci A Calaxin Gene in the Pacific Oyster Crassostrea gigasand Its Potential Roles in Cilia. | ||
Alzheimers Res Ther Uncovering the epigenetic regulatory clues of PRRT1 in Alzheimer's disease: a strategy integrating multi-omics analysis with explainable machine learning
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