验证数据展示
经过测试的应用
| Positive WB detected in | K-562 cells, MCF-7 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
产品信息
14101-1-AP targets PRR13 in WB, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human |
| 文献引用反应性 | human |
| 免疫原 |
CatNo: Ag5280 Product name: Recombinant human PRR13 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-148 aa of BC066943 Sequence: MWNPNAGQPGPNPYPPNIGCPGGSNPAHPPPINPPFPPGPCPPPPGAPHGNPAFPPGGPPHPVPQPGYPGCQLLGPYPPPYPPPAPGIPPVNPLAPGMVGPAVIVDKKMQKKMKKAHKKMHKHQKHHKYHKHGKHSSSSSSSSSSDSD 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | proline rich 13 |
| 别名 | proline rich 13, Proline rich protein 13, PRR13, Taxane resistance protein, TXR1 |
| 计算分子量 | 15.2 kDa |
| 观测分子量 | ~20 kDa |
| GenBank蛋白编号 | BC066943 |
| 基因名称 | PRR13 |
| Gene ID (NCBI) | 54458 |
| RRID | AB_2878012 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9NZ81 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
PRR13, also named as TXR1, is reported to be a key regulator of the resistance to cytostatica by decreasing the copy number of the proapoptotic gene thrombospondin-1. PRR13 has some isoforms with MW 10 kDa, 15-17 kDa and 19-23 kDa.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for PRR13 antibody 14101-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
