验证数据展示
经过测试的应用
| Positive WB detected in | A549 cells, HeLa cells, L02 cells, MCF-7 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:5000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
27422-1-AP targets PRR11 in WB, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 | PRR11 fusion protein Ag26636 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | proline rich 11 |
| 别名 | Proline rich protein 11, proline rich 11 |
| 计算分子量 | 360 aa, 40 kDa |
| 观测分子量 | 38 kDa |
| GenBank蛋白编号 | BC008669 |
| 基因名称 | PRR11 |
| Gene ID (NCBI) | 55771 |
| RRID | AB_3669600 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q96HE9 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
PRR11, or Proline-rich protein 11, is a protein-coding gene situated on chromosome 17q22-23, which plays a significant role in various cellular processes. The protein itself is composed of a nuclear localization signal (NLS), two proline-rich regions (PRs), and a zinc finger domain (ZFD) (PMID: 36551227). PRR11 has been implicated in the regulation of cell cycle, and it is involved in multiple aspects of cancer biology, including proliferation, migration, invasion, cell-cycle progression, apoptosis, autophagy, and chemotherapy resistance. It serves as a prognostic indicator in certain human cancers and is considered a potential target for therapeutic interventions (PMID: 36739300).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for PRR11 antibody 27422-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |