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验证数据展示

  • WB analysis using 68780-1-Ig (same clone as 68780-1-PBS)

    WB analysis using 68780-1-Ig (same clone as 68780-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68780-1-Ig (PRPH2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68780-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP50146-1

    Cytometric bead array standard curve of MP50146-1

    Cytometric bead array standard curve of MP50146-1, PRPH2 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 68780-1-PBS. Detection antibody: 68780-2-PBS. Standard:Ag12555. Range: 1.563-100 ng/mL.

  • Affinity and Kinetic Characterization of 68780-1-Ig

    Affinity and Kinetic Characterization of 68780-1-Ig

    Biolayer interferometry (BLl) kinetic assay of 68780-1-Ig against Human PRPH2 was performed. The affinity constant is below 1 pM.

PRPH2 Monoclonal antibody, PBS Only (Capture)

PRPH2 Monoclonal Antibody for WB, Cytometric bead array, Indirect ELISA
Cat No. 68780-1-PBS

产品说明书

CloneNo. 2F11A1

宿主/亚型

Mouse / IgG2a

种属反应性

human, mouse, rat, rabbit

应用

WB, Cytometric bead array, Indirect ELISA

别名

2F11A1, AOFMD, AVMD, Peripherin 2, Peripherin2

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

68780-1-PBS targets PRPH2 as part of a matched antibody pair:

MP50146-1: 68780-1-PBS capture and 68780-2-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, Cytometric bead array, Indirect ELISA Application Description
经测试反应性 human, mouse, rat, rabbit
免疫原

CatNo: Ag12555

Product name: Recombinant human PRPH2 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 124-346 aa of BC074720

Sequence: GSLENTLGQGLKNGMKYYRDTDTPGRCFMKKTIDMLQIEFKCCGNNGFRDWFEIQWISNRYLDFSSKEVKDRIKSNVDGRYLVDGVPFSCCNPSSPRPCIQYQITNNSAHYSYDHQTEELNLWVRGCRAALLSYYSSLMNSMGVVTLLIWLFEVTITIGLRYLQTSLDGVSNPEESESESEGWLLEKSVPETWKAFLESVKKLGKGNQVEAEGAGAGQAPEAG

种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 peripherin 2 (retinal degeneration, slow)
别名 2F11A1, AOFMD, AVMD, Peripherin 2, Peripherin2
计算分子量 346 aa, 39 kDa
观测分子量35-39 kDa
GenBank蛋白编号BC074720
基因名称 PRPH2
Gene ID (NCBI) 5961
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP23942
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Peripherin-2 (PRPH2), also known as retinal degeneration slow protein (RDS), is a photoreceptor-specific tetraspanin protein implicated in outer segment disk morphogenesis. It may function as an adhesion molecule involved in stabilization and compaction of outer segment disks or in the maintenance of the curvature of the rim. Mutations in peripherin-2 are responsible for various retinal degenerative diseases including autosomal dominant retinitis pigmentosa (ADRP).