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Peripherin Polyclonal antibody

Peripherin Polyclonal Antibody for FC, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

FC, IHC, WB, ELISA and More (1)

Conjugate

Unconjugated

Cat No : 17399-1-AP

Print datasheet

Synonyms

peripherin, PRPH, PRPH1

验证数据展示

  • WB analysis using 17399-1-AP

    WB analysis using 17399-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 17399-1-AP (Peripherin antibody) at dilution of 1:120000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse brain using 17399-1-AP

    IHC staining of mouse brain using 17399-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17399-1-AP (Peripherin antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • WB analysis of mouse colon using 17399-1-AP

    WB analysis of mouse colon using 17399-1-AP

    mouse colon tissue were subjected to SDS PAGE followed by western blot with 17399-1-AP (PRPH antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of rat brain using 17399-1-AP

    IHC staining of rat brain using 17399-1-AP

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 17399-1-AP (Peripherin antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • FC experiment of PC-12 using 17399-1-AP

    FC experiment of PC-12 using 17399-1-AP

    1X10^6 PC-12 cells were intracellularly stained with 0.4 ug Anti-Human Peripherin (17399-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive WB detected inmouse colon tissue, COLO 320 cells, human colon tissue, rat colon tissue, rat spinal cord tissue
Positive IHC detected inmouse brain tissue, human colon tissue, human brain tissue, rat brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected inPC-12 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:20000-1:100000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IFSee 1 publications below

产品信息

17399-1-AP targets Peripherin in WB, IF, FC, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications FC, IHC, WB, ELISA
Cited ApplicationsIF
Tested Reactivity human, mouse, rat
Cited Reactivityrat
Immunogen Peripherin fusion protein Ag11365 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name peripherin
Synonyms peripherin, PRPH, PRPH1
Calculated Molecular Weight 470 aa, 54 kDa
Observed Molecular Weight 54-60 kDa
GenBank Accession NumberBC032703
Gene Symbol PRPH
Gene ID (NCBI) 5630
RRIDAB_2171195
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP41219
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Peripherin is a neuron-specific intermediate filament protein that is particularly expressed in peripheral nervous system. It is used to study peripheral nerve development and regeneration, as it is highly expressed during elongation of axon. Peripherin is a routine marker for unmyelinated neurons in trigeminal ganglion (TG) and the dorsal root ganglion (DRG). Peripherin is also expressed in peri-insular areas of islets and it has been identified as a key autoantigen for type 1A diabetes.

实验方案

Product Specific Protocols
WB protocol for Peripherin antibody 17399-1-APDownload protocol
IHC protocol for Peripherin antibody 17399-1-APDownload protocol
FC protocol for Peripherin antibody 17399-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
ratIF

Biotechniques

Antibody incubation at 37°C improves fluorescent immunolabeling in free-floating thick tissue sections.

Authors - Xia Xiao
View Article