验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, HEK-293 cells, U-251 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
86123-1-RR targets PRPF6 in WB, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 | PRPF6 fusion protein Ag13817 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | PRP6 pre-mRNA processing factor 6 homolog (S. cerevisiae) |
| 别名 | Androgen receptor N-terminal domain-transactivating protein 1, ANT 1, ANT-1, C20orf14, hPrp6 |
| 计算分子量 | 941 aa, 107 kDa |
| 观测分子量 | 100-108 kDa |
| GenBank蛋白编号 | BC001666 |
| 基因名称 | PRPF6 |
| Gene ID (NCBI) | 24148 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | O94906 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
PRPF6 is involved in pre-mRNA splicing as component of the U4/U6-U5 tri-snRNP complex, it can enhance dihydrotestosterone-induced transactivation activity of AR, as well as dexamethasone-induced transactivation activity of NR3C1, but does not affect estrogen-induced transactivation.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for PRPF6 antibody 86123-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |