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验证数据展示

  • WB analysis of Y79 using 16929-1-AP

    WB analysis of Y79 using 16929-1-AP

    WB result of PRPF40B antibody (16929-1-AP; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PRPF40B transfected Y79 cells.

  • IP experiment of Y79 using 16929-1-AP

    IP experiment of Y79 using 16929-1-AP

    IP result of anti-PRPF40B (IP:16929-1-AP, 4ug; Detection:16929-1-AP 1:5000) with Y79 cells lysate 2800 ug.

  • WB analysis using 16929-1-AP

    WB analysis using 16929-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16929-1-AP (PRPF40B antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Featured Product
  • KD/KO验证

PRPF40B Polyclonal antibody

PRPF40B Polyclonal Antibody for WB, IP, ELISA
Cat No. 16929-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IP, ELISA and More (1)

别名

Huntingtin yeast partner C, HYPC, PRPF40B

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inY79 cells, K-562 cells, mouse testis tissue
Positive IP detected inY79 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:12000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IFSee 1 publications below

产品信息

16929-1-AP targets PRPF40B in WB, IF, IP, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IP, ELISA Application Description
文献引用应用IF
经测试反应性 human, mouse
文献引用反应性human
免疫原

CatNo: Ag10361

Product name: Recombinant human PRPF40B protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 522-871 aa of BC067364

Sequence: AVSTDVRFANMLGQPGSTPLDLFKFYVEELKARFHDEKKIIKDILKDRGFCVEVNTAFEDFAHVISFDKRAAALDAGNIKLTFNSLLEKAEAREREREKEEARRMRRREAAFRSMLRQAVPALELGTAWEEVRERFVCDSAFEQITLESERIRLFREFLQVLEQTECQHLHTKGRKHGRKGKKHHHKRSHSPSGSESEEEELPPPSLRPPKRRRRNPSESGSEPSSSLDSVESGGAALGGRGSPSSHLLGADHGLRKAKKPKKKTKKRRHKSNSPESETDPEEKAGKESDEKEQEQDKDRELQQAELPNRSPGFGIKKEKTGWDTSESELSEGELERRRRTLLQQLDDHQ

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 PRP40 pre-mRNA processing factor 40 homolog B (S. cerevisiae)
别名 Huntingtin yeast partner C, HYPC, PRPF40B
计算分子量 871 aa, 99 kDa
观测分子量130-150 kDa
GenBank蛋白编号BC067364
基因名称 PRPF40B
Gene ID (NCBI) 25766
RRIDAB_2171041
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ6NWY9
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IP protocol for PRPF40B antibody 16929-1-APDownload protocol
WB protocol for PRPF40B antibody 16929-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIF

Nucleic Acids Res

PRPF40A induces inclusion of exons in GC-rich regions important for human myeloid cell differentiation

Authors - Cheryl Weiqi Tan
View Article
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