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验证数据展示

  • WB analysis of K-562 using 24480-1-AP

    WB analysis of K-562 using 24480-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 24480-1-AP (PRPF39 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human testis using 24480-1-AP

    IHC staining of human testis using 24480-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 24480-1-AP (PRPF39 Antibody) at dilution of 1:400 (under 40x lens).

PRPF39 Polyclonal antibody

PRPF39 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 24480-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, ELISA

别名

Pre mRNA processing factor 39, PRP39 homolog, PRPF39

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inK-562 cells, COLO 320 cells, Raji cells
Positive IHC detected inhuman testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

24480-1-AP targets PRPF39 in WB, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human
免疫原 PRPF39 fusion protein Ag21683 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 PRP39 pre-mRNA processing factor 39 homolog (S. cerevisiae)
别名 Pre mRNA processing factor 39, PRP39 homolog, PRPF39
计算分子量 669 aa, 78 kDa
观测分子量 75-85 kDa
GenBank蛋白编号BC125126
基因名称 PRPF39
Gene ID (NCBI) 55015
RRIDAB_2879563
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ86UA1
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PRPF39 also termed as Pre mRNA processing factor 39 or PRP39 homolog is a 669 amino acid protein, which contains 7 HAT repeats and belongs to the PRP39 family. PRPF39 localizes in nucleus and may play a role in mRNA splicing.

实验方案

Product Specific Protocols
WB protocol for PRPF39 antibody 24480-1-APDownload protocol
IHC protocol for PRPF39 antibody 24480-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols