PRPF3 Polyclonal antibody
PRPF3 Polyclonal Antibody for IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
IP, WB, ELISA and More (1)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
Positive WB detected in | Y79 cells, HepG2 cells, L02 cells, mouse brain tissue, mouse liver tissue |
Positive IP detected in | mouse liver tissue |
推荐稀释比
Application | Dilution |
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Western Blot (WB) | WB : 1:500-1:2000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
WB | See 3 publications below |
IF | See 1 publications below |
产品信息
10106-1-AP targets PRPF3 in WB, IP, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Applications | IP, WB, ELISA |
Cited Applications | WB, IF |
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human |
Immunogen | PRPF3 fusion protein Ag0154 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | PRP3 pre-mRNA processing factor 3 homolog (S. cerevisiae) |
Synonyms | HPRP3, HPRP3P, Pre mRNA splicing factor 3, PRP3, Prp3p, PRPF3, RP18, U4/U6 snRNP 90 kDa protein |
Calculated Molecular Weight | 78 kDa |
Observed Molecular Weight | 90 kDa, 78 kDa |
GenBank Accession Number | BC000184 |
Gene Symbol | PRPF3 |
Gene ID (NCBI) | 9129 |
RRID | AB_2170994 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | O43395 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
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WB protocol for PRPF3 antibody 10106-1-AP | Download protocol |
IP protocol for PRPF3 antibody 10106-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Hum Mol Genet PRPF mutations are associated with generalized defects in spliceosome formation and pre-mRNA splicing in patients with retinitis pigmentosa. | ||
Mol Cell Biol A quantitative high-throughput in vitro splicing assay identifies inhibitors of spliceosome catalysis. | ||
J Biol Chem PRPF19 regulates p53-dependent cellular senescence by modulating alternative splicing of MDM4 mRNA. | ||
Cell Mol Biol Lett TMEM43 promotes pancreatic cancer progression by stabilizing PRPF3 and regulating RAP2B/ERK axis. |