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验证数据展示

  • IP experiment of Jurkat using 31494-1-AP

    IP experiment of Jurkat using 31494-1-AP

    IP result of anti-C13orf23 (IP:31494-1-AP, 4ug; Detection:31494-1-AP 1:500) with Jurkat cells lysate 1800 ug.

  • IHC staining of human ovary tumor using 31494-1-AP

    IHC staining of human ovary tumor using 31494-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 31494-1-AP (C13orf23 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of U2OS using 31494-1-AP

    IF Staining of U2OS using 31494-1-AP

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using PROSER1 antibody (31494-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

PROSER1 Polyclonal antibody

PROSER1 Polyclonal Antibody for IHC, IF/ICC, IP, ELISA
Cat No. 31494-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IHC, IF/ICC, IP, ELISA

别名

C13orf23, bA50D16.2, KIAA2032, Proline And Serine Rich 1, Proline and serine-rich protein 1

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive IP detected inJurkat cells
Positive IHC detected inhuman ovary tumor tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inU2OS cells

推荐稀释比

应用推荐稀释比
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

31494-1-AP targets PROSER1 in IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

经测试应用 IHC, IF/ICC, IP, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag35678

Product name: Recombinant human C13orf23 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-178 aa of NM_025138.4

Sequence: MDKKSFEMVLDEIRKAVLTEYKLKAIEYVHGYFSSEQVVDLLRYFSWAEPQLKAMKALQHKMVAVQPTEVVNILNCFTFSKDKLVALELLASNIIDAQNSRPIEDLFRVNMSEKKRCKRILEQAFKGGCKAPHAMISSCGTIPGNPYPKGRPSRINGIFPGTPLKKDGEECTNEGKGI

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 chromosome 13 open reading frame 23
别名 C13orf23, bA50D16.2, KIAA2032, Proline And Serine Rich 1, Proline and serine-rich protein 1
计算分子量96kDa，944aa
观测分子量100 kDa
GenBank蛋白编号NM_025138.4
基因名称 C13orf23
Gene ID (NCBI) 80209
RRIDAB_3742438
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity Purification
UNIPROT IDQ86XN7
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PROSER1 (proline- and serine-rich 1) is a chromatin-binding protein encoded by the 13q13.3 locus. It interacts with all three TET enzymes and serves as the core scaffold of the TOPD (TET‑OGT‑PROSER1‑DBHS) complex, stabilizing the binding between TET2 and OGT while promoting O‑GlcNAcylation, thereby enhancing DNA demethylation activity. PROSER1 has recently been found to be associated with neurodevelopmental disorders.

实验方案

Product Specific Protocols
IF protocol for PROSER1 antibody 31494-1-APDownload protocol
IHC protocol for PROSER1 antibody 31494-1-APDownload protocol
IP protocol for PROSER1 antibody 31494-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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