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验证数据展示

  • WB analysis using 25154-1-AP

    WB analysis using 25154-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 25154-1-AP (PROSC antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IHC staining of human skeletal muscle using 25154-1-AP

    IHC staining of human skeletal muscle using 25154-1-AP

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 25154-1-AP (PROSC Antibody) at dilution of 1:200 (under 10x lens).

  • IHC staining of human skeletal muscle using 25154-1-AP

    IHC staining of human skeletal muscle using 25154-1-AP

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 25154-1-AP (PROSC Antibody) at dilution of 1:200 (under 40x lens).

  • IF Staining of U2OS using 25154-1-AP

    IF Staining of U2OS using 25154-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed U2OS cells using PROSC antibody (25154-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

PROSC Polyclonal antibody

PROSC Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 25154-1-AP

产品说明书

引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

human and More (1)

应用

WB, IHC, IF/ICC, ELISA

别名

PLPBP, PLP homeostasis protein, Proline synthase co-transcribed bacterial homolog protein, Pyridoxal phosphate homeostasis protein, Pyridoxal phosphate-binding protein

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHepG2 cells, L02 cells
Positive IHC detected inhuman skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inU2OS cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:100-1:400
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 2 publications below

产品信息

25154-1-AP targets PROSC in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
文献引用应用WB
经测试反应性 human
文献引用反应性human, mouse
免疫原 PROSC fusion protein Ag18369 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 proline synthetase co-transcribed homolog (bacterial)
别名 PLPBP, PLP homeostasis protein, Proline synthase co-transcribed bacterial homolog protein, Pyridoxal phosphate homeostasis protein, Pyridoxal phosphate-binding protein
计算分子量 275 aa, 30 kDa
观测分子量 30 kDa
GenBank蛋白编号BC012334
基因名称 PROSC
Gene ID (NCBI) 11212
RRIDAB_2879929
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDO94903
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Human Pyridoxal 5′-phosphate-binding protein (PLPBP; previously proline synthase cotranscribed homolog, PROSC) was first described in 1999 and was demonstrated to be ubiquitously expressed in many tissues. PROSC is ubiquitously expressed in human tissues and is highly conserved throughout evolution. PROSC deficiency could therefore markedly increase free cellular PLP levels (PMID: 31825581, 27912044).

实验方案

Product Specific Protocols
WB protocol for PROSC antibody 25154-1-APDownload protocol
IHC protocol for PROSC antibody 25154-1-APDownload protocol
IF protocol for PROSC antibody 25154-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Brain

PLPHP deficiency: clinical, genetic, biochemical, and mechanistic insights.

Authors - Devon L Johnstone
View Article
mouseWB

Int J Mol Sci

Translation Fidelity and Respiration Deficits in CLPP-Deficient Tissues: Mechanistic Insights from Mitochondrial Complexome Profiling

Authors - Jana Key
View Article