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验证数据展示

  • WB analysis using 18739-1-AP

    WB analysis using 18739-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 18739-1-AP (PROP1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IHC staining of human placenta using 18739-1-AP

    IHC staining of human placenta using 18739-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 18739-1-AP (PROP1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human placenta using 18739-1-AP

    IHC staining of human placenta using 18739-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 18739-1-AP (PROP1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

PROP1 Polyclonal antibody

PROP1 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 18739-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, ELISA

别名

Homeobox protein prophet of Pit-1, Pituitary-specific homeodomain factor, PROP 1, PROP paired like homeobox 1, PROP-1

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inhuman placenta tissue, mouse embryo
Positive IHC detected inhuman placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

18739-1-AP targets PROP1 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse
免疫原

Peptide

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 PROP paired-like homeobox 1
别名 Homeobox protein prophet of Pit-1, Pituitary-specific homeodomain factor, PROP 1, PROP paired like homeobox 1, PROP-1
计算分子量 25 kDa
观测分子量25 kDa
GenBank蛋白编号BC069076
基因名称 PROP1
Gene ID (NCBI) 5626
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDO75360
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PROP1 belongs to the paired homeobox family. It contains one homeobox DNA-binding domain. PROP1 is possibly involved in the ontogenesis of pituitary gonadotropes, as well as somatotropes, lactotropes and caudomedial thyrotropes. It is expressed specifically in embryonic pituitary. Mutation of PROP1 will cause combined pituitary hormone deficiency (CPHD) which is characterized by impaired production of growth hormone (GH) and one or more of the other five anterior pituitary hormones.

实验方案

Product Specific Protocols
IHC protocol for PROP1 antibody 18739-1-APDownload protocol
WB protocol for PROP1 antibody 18739-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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